Ghrelin及其相关基因mRNA在海兰褐蛋鸡不同产蛋阶段的表达变化

发布时间：2019-08-14 09:53

【摘要】：本文旨在探讨海兰褐蛋鸡Ghrelin、Ghrelin受体(GHSR)和Ghrelin酰基转移酶(GOAT)在产蛋周期不同阶段的表达变化规律,为通过诱导Ghrelin表达促进蛋鸡卵泡生长发育以提高产蛋率及延长产蛋周期提供理论依据。应用ELISA检测蛋鸡产蛋周期血浆和卵巢Ghrelin含量,实时定量PCR检测腺胃与卵巢Ghrelin、GHSR和GOAT表达变化。结果表明:不同产蛋阶段外周血Ghrelin的含量和腺胃中Ghrelin、GHSR和GOAT mRNA的表达变化不明显;产蛋高峰期卵巢中Ghrelin mRNA表达水平比产蛋前期升高98.2%(P0.05),而产蛋后期Ghrelin表达水平比产蛋高峰期有所下降(P0.05);GHSR和GOAT的表达模式和Ghrelin基本一致,均表现为产蛋高峰期表达量最高,产蛋后期略有下降,产蛋高峰期GHSR和GOAT mRNA表达水平显著高于产蛋前期和产蛋后期(P0.05),但产蛋前期和后期差异不显著(P0.05)。推测海兰褐蛋鸡卵巢中Ghrelin、GHSR和GOAT的转录表达水平与产蛋周期具有一定的相关性。
[Abstract]:The purpose of this study was to investigate the expression of Ghrelin,Ghrelin receptor (GHSR) and Ghrelin acyltransferase (GOAT) in different stages of laying cycle in Hailan brown laying hens, and to provide theoretical basis for promoting follicular growth and development of laying hens by inducing Ghrelin expression to increase laying rate and prolong laying cycle. The contents of Ghrelin in plasma and ovary of laying hens were detected by ELISA, and the expression of Ghrelin,GHSR and GOAT in adenogastric and ovarian were detected by real-time quantitative PCR. The results showed that the content of Ghrelin in peripheral blood and the expression of Ghrelin,GHSR and GOAT mRNA in adenogastric stomach did not change significantly at different laying stages, the expression level of Ghrelin mRNA in ovary during laying period was 98.2% higher than that in pre-laying stage (P 0.05), while the expression level of Ghrelin in late laying stage was lower than that in egg laying peak period (P 0.05). The expression pattern of GHSR and GOAT was basically the same as that of Ghrelin, both of which showed the highest expression at the peak period of laying eggs and a slight decrease at the late stage of laying eggs. The expression levels of GHSR and GOAT mRNA in the peak period of laying eggs were significantly higher than those in the early and late stages of laying eggs (P 0.05), but there was no significant difference between the early and late stages of laying eggs (P 0.05). It is speculated that the transcriptional expression of Ghrelin,GHSR and GOAT in the ovaries of Hailan brown laying hens is related to the laying cycle.
【作者单位】：河南科技学院动物科技学院;
【基金】：河南省高校科技创新团队项目(18IRTSTHN019) 河南省科技攻关项目(162102110038) 新乡市科技攻关项目(CXGG16026)
【分类号】：S831

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