The Effect of PHF20, CYHR1, LOC514211, MAN1A1, Spatc1 Gene M

发布时间：2022-02-11 17:35

　　The objective of this study were to identify and confirm the SNP associated with milk production and reproduction, whose SNP were selected from Chinese and British dairy cows based on 60k SNP-Chip. The research was conducted in laboratory of animal genetics, breeding and reproduction in Huazhong Agricultural University, Wuhan City, Hubei Province, People’s Republic of China, starts on March 2010-December 2012.470 head Dairy Cows and 35 head water buffaloes have been used in this research, DNA wa... 

【文章来源】：华中农业大学湖北省211工程院校教育部直属院校

【文章页数】：68 页

【学位级别】：硕士

【文章目录】：
ABSTRACT
Abbreviation
CHAPTER Ⅰ INTRODUCTION
CHAPTER Ⅱ LITERATURE REVIEW
    2.1 Water Buffalo and Dairy Cow
    2.2 Candidate Gene
        2.2.1 PHF 20 Gene
        2.2.2 CYHR 1 Gene
        2.2.3 LOC 514211 Gene
        2.2.4 MAN1A1 Gene
        2.2.5 SPATC1 Gene
    2.3 Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP)
    2.4 Single Nucleotide Polymorphism (SNP)
    2.5 SNP Association Studies in Milk production
    2.6 SNP Association Studies in Reproduction
CHAPTER Ⅲ MATERIAL AND METHODE
    3.1 Material Research
        3.1.1 DNA Samples
        3.1.2 Equipment and Supplies
        3.1.3 Reagents and Solutions
    3.2 Method
        3.2.1 Blood sample
        3.2.2 DNA extraction
        3.2.3 SNP Detection and genotyping
    3.3 Statistical Analysis
CHAPTER Ⅳ RESULTS AND DISCUSSION
    4.1 Identification genotype of SNPs of Candidate Genes in Dairy Cow and Water Buffalo
        4.1.1 PHF20 rs 43713830
            4.1.1.1 Genotype of SNP in Dairy Cow
            4.1.1.2 Genotypes Frequency of SNP in Dairy Cow
            4.1.1.3 Genotypes and Frequency of SNP in Water Buffalo
        4.1.2 CYHR1 rs 109968515
            4.1.2.1 Genotypes of SNP in Dairy Cow
            4.1.2.2 Genotypes Frequency of SNP in Dairy Cow
            4.1.2.3 Genotypes and Frequency of SNP in Water Buffalo
        4.1.3 LOC514211 rs 42688595
            4.1.3.1 Genotypes of SNP in Dairy Cow
            4.1.3.2 Genotypes Frequency of SNP in Dairy Cow
            4.1.3.3 Genotypes and Frequency of SNP in Water Buffalo
        4.1.4 MAN1A1 rs 42855643
            4.1.4.1 Genotypes of SNP in Dairy Cow
            4.1.4.2 Genotypes Frequency of SNP in Dairy Cow
            4.1.4.3 Genotypes and Frequency of SNP in Water Buffalo
        4.1.5 SPATC1 rs 41629750
            4.1.5.1 Genotypes of SNP in Dairy Cow
            4.1.5.2 Genotypes Frequency of SNP in Dairy Cow
            4.1.5.3 Genotypes and Frequency of SNP in Water Buffalo
    4.2 Association Analysis of SNP Candidate Gene with Milk Yield in Dairy Cow
        4.2.1 Association Analysis of SNP Candidate Gene with 305 Days with Milk (305 DM)
        4.2.2 Association Analysis of SNP Candidate Gene with Total Milk Yield
    4.3 Analysis Association of SNP Candidate Gene with Milk Quality in Dairy Cow
        4.3.1 Analysis Association of SNP Candidate Gene with Fat Milk Rate
        4.3.2. Analysis Association of SNP Candidate Gene with Protein Milk Rate
        4.3.3 Analysis Association of SNP Candidate Gene with Total Fat Yield
        4.3.4 Analysis Association of SNP Candidate Gene with Total Protein Yield
    4.4 Analysis Association of SNP Candidate Gene with Reproductive Traits in Dairy Cow
        4.4.1. Analysis Association of SNP Candidate Gene with Days to First Service
        4.4.2. Analysis Association of SNP Candidate Gene with Days Open
        4.4.3 Analysis Association of SNP Candidate Gene with Birth Weight
        4.4.4 Analysis Association of SNP Candidate Gene with Calving Interval
        4.4.5. Analysis Association of SNP Candidate Gene with Age of Calving
    4.5 Analysis Association of SNP Candidate Gene with Milk Production and Reproductionin Water Buffalo
CHAPTER Ⅴ DISCUSSION
    5.1 Identification genotype of SNPs of Candidate Genes in Dairy Cow and Water Buffalo
    5.2 Association Analysis of SNP Candidate Gene with Milk Yield in Dairy Cow
    5.3 Association Analysis of SNP Candidate Gene with Milk Quality in Dairy Cow
    5.4 Association Analysis of SNP Candidate Gene with Reproduction in Dairy Cow
    5.5 Analysis of SNP Candidate Gene with Milk Production and Reproductive Trait in Water Buffalo
CHAPTER Ⅵ CONCLUSION
CHAPTER Ⅶ FURTHER RESEARCH
LITERATURE CITED
ACKNOWLEDGEMENTS


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期刊论文
[1]辽西地区奶牛乳房炎发病率的调查及其综合防治措施的探讨[J]. 陶青,于明鑫,赵玉辉,王东星,刘旭.  中国牛业科学. 2007(04)



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