性激素结合球蛋白和葡萄糖转运蛋白在妊娠期糖尿病胎盘组织中的表达及相关性

发布时间：2018-01-25 09:42

本文关键词： 性激素结合球蛋白葡萄糖转运蛋白类胎盘妊娠期糖尿病　出处：《中国医科大学学报》2015年01期 　论文类型：期刊论文

【摘要】：目的探讨妊娠期糖尿病(GDM)胎盘组织细胞中性激素结合球蛋白(SHBG)和葡萄糖转运蛋白(GLUT1、GLUT3、GLUT4)的表达及相关性。方法应用免疫细胞化学染色法检测SHBG与GLUT1、GLUT3、GLUT4在30例GDM孕妇和30例正常孕妇胎盘组织中的表达情况。结果在GDM孕妇胎盘组织中,SHBG、GLUT1、GLUT3、GLUT4阳性细胞的平均光密度值分别为0.401 6±0.014 8,0.405 8±0.012 3,0.402 0±0.012 1,0.391 3±0.008 4。在正常孕妇胎盘组织中,阳性细胞的平均光密度值分别为0.414 2±0.011 5,0.427 0±0.029 4,0.418 0±0.008 7,0.402 9±0.095 9。比较SHBG与GLUT1、GLUT3、GLUT4在GDM孕妇和正常孕妇胎盘组织中的表达水平,差异均有统计学意义(P0.05)。Pearson相关分析结果显示:SHBG和GLUT4在胎盘组织中的表达量呈正相关(R2=0.410 4,P0.05)。结论 GLUT1是胎盘中最主要的葡萄糖转运载体,可能在母婴间葡萄糖转运中发挥主要的作用。GLUT4在胎盘组织表达较少,却受SHBG表达的影响。
[Abstract]:Objective to investigate the gestational diabetes mellitus (GDM) in placenta cells of sex hormone binding globulin (SHBG) and glucose transporter (GLUT1, GLUT3, GLUT4) expression and correlation method. Immunocytochemical staining was used to detect SHBG and GLUT1 expression of GLUT4, GLUT3, GDM in 30 cases of pregnant women and 30 cases of normal pregnancy women tissue. Results in the placenta of GDM, SHBG, GLUT1, GLUT3, the average optical density of GLUT4 positive cells were 0.4016 + 0.014 8,0.405 8 + 0.012 3,0.402 0 + 0.012 1,0.391 3 + 0.008 and 4. in normal placenta, the average optical density of positive cells was 0.4142 + 0.011 5,0.427 0 + 0.029 4,0.418 0 + 0.008 7,0.402 9 + 0.095 9. SHBG compared with GLUT1, GLUT3, GDM expression level of GLUT4 in pregnant women and normal pregnant women in the placenta, the differences were statistically significant (P0.05).Pearson correlation analysis showed that The expression level of SHBG and GLUT4 in placenta tissue is positively correlated (R2=0.410 4, P0.05). Conclusion GLUT1 is the most important glucose transporter in placenta, which may play a major role in glucose transport between mother and infant..GLUT4 is less expressed in placenta tissue but is affected by SHBG expression.

【作者单位】：中国医科大学附属盛京医院妇产科;
【基金】：国家自然科学基金(81170591;81300511) 沈阳市科学技术计划项目(F11-262-9-46)
【分类号】：R714.256
【正文快照】： 最近的研究表明,性激素结合球蛋白(sex hor-mone binding globulin,SHBG)在胎盘等组织中有不同程度的表达。SHBG除能特异性的结合并转运性激素外,还具有介导激素信号传导和类激素样作用。而且,SHBG是糖尿病的危险因素之一,在妊娠期糖尿病(gestational diabetes mellitus,GDM)

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