CD1a、CD83在药物流产后异常子宫出血组织中的表达及意义

发布时间：2018-05-03 14:09

本文选题：树突状细胞 + 药物流产　；参考：《现代预防医学》2015年20期

【摘要】：目的通过对树突状细胞(Dendritic Cells,DCs)表面标志物CD1a、CD83在异常子宫出血组织中表达状况的研究,探讨其在药物流产后异常子宫出血中的作用。方法选取自愿终止妊娠的停经8周之内的早孕妇女,分为人工流产组、药流不全组、药流完全组各20例。收集3组蜕膜组织应用免疫组化法和Western blotting法检测不同分化阶段DCs的分布、形态、数量。结果 3组DCs均分布于腺体间、血管间;CD1a+未成熟DCs呈圆形、卵圆形;CD83+成熟DCs有多个分枝状突起。人流组CD1a的表达较药流组高,但CD83的表达较药流组低,差异有统计学意义(P0.01);药流不全组CD1a的表达较药流全组高,但CD83的表达较药流全组低,差异有统计学意义(P0.01)。结论不同分化阶段DCs与药物流产后异常子宫出血的发生发展有密切相关性。
[Abstract]:Objective to study the expression of CD1a- CD83, a surface marker of dendritic cells, in abnormal uterine bleeding and its role in abnormal uterine bleeding after drug abortion. Methods early pregnant women who voluntarily terminated pregnancy within 8 weeks were divided into three groups: induced abortion group (n = 20), incomplete abortion group (n = 20) and complete drug flow group (n = 20). Three groups of decidual tissues were collected to detect the distribution, morphology and quantity of DCs in different differentiation stages by immunohistochemical method and Western blotting method. Results the DCs of the three groups were distributed between glands. The immature DCs of CD1a was round, and the mature DCs of CD83 had multiple branching processes. The expression of CD1a was higher in the artificial flow group than in the drug flow group, but the expression of CD83 was lower than that in the drug flow group, the difference was statistically significant (P 0.01), and the expression of CD1a in the drug flow insufficiency group was higher than that in the whole drug flow group, but the expression of CD83 was lower than that in the whole drug flow group, and the difference was statistically significant (P 0.01). Conclusion DCs in different differentiation stages is closely related to the occurrence and development of abnormal uterine bleeding after drug abortion.
【作者单位】：华北理工大学组织胚胎学系;华北理工大学办公室;华北理工大学心理心理学院;
【基金】：河北省科学技术研究与发展计划自筹经费项目课题(12277747) 河北省科技支撑项目(13277726D) 唐山市科学技术研究与发展计划项目资助课题(12150222B-16)
【分类号】：R169.42

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