肿瘤相关巨噬细胞M2与宫颈鳞状细胞癌浸润类型关系的初步研究

发布时间：2018-05-19 10:12

  本文选题：宫颈鳞状细胞癌 + 浸润类型　； 参考：《宁夏医科大学》2017年硕士论文

【摘要】：目的宫颈癌(cervical cancer,CC)是女性常见的癌症之一,为全球女性最常见癌症的第二位,女性恶性肿瘤死亡率排行第三位,在发展中国家,是癌症死亡第二个最常见的原因。其中,宫颈鳞状细胞癌(squamous cell carcinoma,SCC)约占宫颈癌的75-80%,宫颈癌的浸润方式及程度很大程度上决定着其预后与治疗。鉴于宫颈SCC比例高且危害极大,急需进一步深刻认识其生物学特征及发生机理,为新的治疗方法提供科学依据。人类多种肿瘤组织的免疫组化研究表明,绝大多数肿瘤相关巨噬细胞M2计数高的肿瘤患者的临床预后较差,因此,研究肿瘤微环境中肿瘤相关巨噬细胞,应当特别关注M2。本课题首次研究了肿瘤相关巨噬细胞M2与宫颈SCC不同浸润方式(invasion patterns)即推边型(pushing border pattern,PBP)和弥漫浸润型(diffuse infiltration pattern,DIP)的关系,为深入理解宫颈SCC的浸润机制提供了新依据。方法购置2张美国Biomax公司宫颈组织芯片,每个组织点直径1.5mm、厚度5微米,其中可用病例为109例宫颈SCC和45例宫颈非癌组织,将109例宫颈SCC按浸润方式分为推边型(PBP)和弥漫浸润型(DIP)。用免疫组织化学方法检测组织芯片的CD163阳性细胞并分别计数。结果(1)宫颈SCC癌巢组织中的M2细胞数为(35.8±28.3),宫颈非癌组织上皮的M2细胞数为(1.2±3.6),两组之间具有非常显著的统计学差异(P=0.000);(2)宫颈SCC间质中M2细胞数为(44.7±29.4),宫颈非癌组织间质的M2细胞数为(10.4±9.2),两组之间具有非常显著的统计学差异(P=0.000);(3)宫颈SCC的两种浸润分型中,PBP癌巢的M2细胞数为(20.5±19.5),与DIP癌巢的M2细胞数(37.7±28.7)相比,具有显著的统计学差异(P=0.046);(4)宫颈SCC的两种浸润分型中,宫颈SCCPBP间质的M2细胞数为(25.1±18.0),与DIP癌间质的M2细胞数(47.1±29.7)相比,具有非常显著的统计学差异(P=0.002)。结论(1)首次发现,宫颈SCC癌巢及间质中的肿瘤相关巨噬细胞M2数与非癌组织相比具有非常显著的统计学差异;(2)首次发现,DIP宫颈SCC的癌巢及间质中的肿瘤相关巨噬细胞M2数显著多于PBP宫颈SCC。这一发现不仅为认识宫颈SCC的浸润机制提供了新依据,而且为宫颈SCC的免疫治疗提供了实验依据。
[Abstract]:Objective Cervical cancer CCC is one of the most common cancers in women, which is the second most common cancer in the world, the third most common cancer mortality in women, and the second most common cause of cancer death in developing countries. Among them, squamous cell carcinoma of cervix squamous cell carcinoma (SCC) accounts for about 75-80% of cervical carcinoma. The invasive mode and degree of cervical cancer largely determine its prognosis and treatment. In view of the high proportion of cervical SCC and great harm, it is urgent to further understand its biological characteristics and pathogenesis, and provide scientific basis for new treatment methods. Immunohistochemical studies of various human tumor tissues show that the majority of tumor patients with high M2 count of tumor-associated macrophages have poor clinical prognosis. Therefore, the study of tumor-associated macrophages in tumor microenvironments should pay special attention to M _ 2. In this study, we first studied the relationship between tumor associated macrophage M2 (M 2) and cervical SCC invasion pattern patterns (P P) and diffusely infiltrated infiltration pattern border (DIP), which provided a new basis for further understanding the infiltration mechanism of cervical SCC. Methods two pieces of cervical tissue microarray of Biomax were purchased. Each tissue point was 1.5mm in diameter and 5 microns in thickness. The available cases were 109 cases of cervical SCC and 45 cases of non-cancerous cervical tissues. According to the invasive pattern, 109 cases of cervical SCC were divided into edge-pushing type and diffuse infiltrating type. CD163 positive cells in tissue microarray were detected by immunohistochemical method and counted separately. Results (1) the number of M2 cells in cervical SCC cancer nest tissue was 35.8 卤28.3 and that in cervical non-cancerous epithelium was 1.2 卤3.6. There was a very significant statistical difference between the two groups (P < 0. 000) the number of M 2 cells in cervical SCC stroma was 44.7 卤29.44.The number of M 2 cells in cervical noncancerous tissue was 44.7 卤29.42a. There was significant statistical difference between the two groups (P < 0. 000). In the two invasive types of cervical SCC, the number of M 2 cells was 20. 5 卤19. 5 and 37.7 卤28. 7 in the cancer nest of DIP. There were significant statistical differences in the number of M2 cells in cervical SCCPBP stroma between two types of cervical SCC (P < 0.01 卤18.0), and there was a very significant statistical difference in the number of M 2 cells between DIP and DIP stroma (47.1 卤29.7). Conclusion 1) first found, The number of tumor-associated macrophages M2 in cervical SCC nests and stroma was significantly higher than that in non-cancerous tissues. (2) it was the first time to find that the number of tumor-associated macrophages M2 in dip cervical SCC and stroma was significantly higher than that in PBP cervix SCC. This discovery not only provides a new basis for understanding the infiltrating mechanism of cervical SCC, but also provides experimental evidence for the immunotherapy of cervical SCC.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33
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本文编号：1909702