淋巴细胞免疫治疗对不孕不育患者封闭抗体的表达及再妊娠的影响

发布时间：2018-05-30 11:18

本文选题：淋巴细胞免疫疗法 + 复发性流产　；参考：《东南大学学报(医学版)》2017年04期

【摘要】：目的:研究淋巴细胞免疫疗法对复发性流产患者的封闭抗体、淋巴细胞亚群及再妊娠的影响。方法:对符合条件的复发性流产患者行淋巴细胞免疫疗法,分别于治疗前后应用ELISA检测患者外周血封闭抗体的表达,免疫散射比浊法测定免疫球蛋白浓度,流式细胞仪检测患者淋巴细胞亚群数量及相关比值,并对患者进行2年的随访,统计患者妊娠或自然流产发生数。结果:淋巴细胞免疫治疗后封闭抗体阳性表达患者例数显著高于治疗前(χ~2=135.67,P0.001);IgM和IgG免疫球蛋白的浓度显著低于治疗前(P0.05);治疗后的CD3~+和CD4~+淋巴细胞数量显著低于治疗前(P0.05),而CD8~+淋巴细胞数量显著高于治疗前(P0.05),治疗后CD4~+/CD8~+的值较治疗前显著减小(P0.05),治疗后CD56~+CD16~+NK细胞数量显著低于治疗前(P0.05)。封闭抗体阳性者自然流产率为10.26%,封闭抗体阴性者为58.33%,差异有统计学意义(χ~2=20.554,P0.05)。结论:淋巴细胞免疫疗法具有增加患者封闭抗体表达、提高妊娠成功率的作用。
[Abstract]:Aim: to study the effects of lymphocyte immunotherapy on blocking antibody lymphocyte subsets and re-pregnancy in recurrent abortion. Methods: lymphocyte immunotherapy was performed on the patients with recurrent abortion. ELISA was used to detect the expression of peripheral blood blocking antibody and immunoglobulin concentration was determined by immunosurgery before and after treatment. The number of lymphocyte subsets and their relative ratios were measured by flow cytometry. The patients were followed up for 2 years to count the incidence of pregnancy or spontaneous abortion. Results: the number of patients with positive expression of blocking antibody after lymphocyte immunotherapy was significantly higher than that before treatment (蠂 ~ 2 + 135.67) P0.001 IgG immunoglobulin was significantly lower than that before treatment, and the number of CD3 ~ and CD4~ lymphocytes after treatment was significantly lower than that before treatment. The number of CD8- lymphocytes was significantly higher than that of P0.05G before treatment. After treatment, the value of CD4 ~ / CD8- ~ decreased significantly compared with that before treatment, and the number of CD56 ~ CD16- NK cells after treatment was significantly lower than that of P0.05A before treatment. The spontaneous abortion rate was 10.26 in the positive group of blocking antibody and 58.33 in the negative group of blocking antibody, the difference was statistically significant (蠂 ~ (2 +) 20.554) (P < 0.05). Conclusion: lymphocyte immunotherapy can increase the expression of blocking antibody and increase the success rate of pregnancy.
【作者单位】：郑州大学附属洛阳中心医院/洛阳市中心医院妇科;
【分类号】：R711.6

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