内质网应激对碳离子辐照宫颈癌HeLa细胞的影响

发布时间：2018-06-07 00:43

本文选题：碳离子辐照 + 二硫苏糖醇　；参考：《原子核物理评论》2015年02期

【摘要】：利用不同剂量的碳离子辐照二硫苏糖醇(2.5 mmol/L)预处理的HeLa细胞,探讨了内质网应激反应对碳离子辐照宫颈癌HeLa细胞的影响。实验发现:与单独辐照组相比,二硫苏糖醇联合碳离子辐照后细胞的存活率下降,而凋亡率增加;二硫苏糖醇联合碳离子辐照加重了碳离子辐照引起的细胞周期阻滞;且联合辐照组的自噬被明显激活。结果表明,持续的内质网应激可改变宫颈癌HeLa细胞对碳离子辐照反应,且二硫苏糖醇可能通过影响HeLa细胞的自噬性细胞死亡通路发挥作用。
[Abstract]:The effects of endoplasmic reticulum stress on HeLa cells pretreated with different doses of carbon ions were studied. The results showed that the survival rate of cells was decreased and the apoptosis rate was increased after irradiation with dithiothreitol combined with carbon ions, the cell cycle arrest induced by carbon ion irradiation was aggravated by dithiothreitol combined with carbon ion irradiation. Moreover, autophagy in combined irradiation group was significantly activated. The results showed that persistent endoplasmic reticulum stress could change the response of cervical cancer HeLa cells to carbon ion irradiation, and dithiothreitol might play a role in the autophagy cell death pathway of HeLa cells.
【作者单位】：中国科学院近代物理研究所;兰州大学;
【基金】：国家自然科学基金资助项目(11105189,11179002)~~
【分类号】：R737.33

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