实时定量荧光PCR法在子宫颈病变患者HPV检测中的应用研究

发布时间：2018-06-13 07:46

  本文选题：宫颈癌 + 人乳头瘤病毒　； 参考：《青海大学》2014年硕士论文

【摘要】：宫颈癌（cervical cancer）是女性生殖系统常见的恶性肿瘤之一，是全球性公共卫生问题。2008年全球新发宫颈癌病例约52.98万，死亡病例25.51万，其中85%新发病例在发展中国家。研究认为，宫颈癌是由宫颈上皮内瘤变（cervical intraepithelial neoplasias,CIN）逐步发展而来的，而宫颈癌的发生、发展又是多种危险因素综合作用的结果，其中通过性传播使宫颈感染高危型人乳头瘤病毒（high-risk human papillomavirus,HR-HPV）是宫颈癌及其癌前病变的高危型致病因素之一。HR-HPV感染时可以通过医疗干预来预防和治愈的，因此，早期发现HR-HPV感染并采取积极干预措施是防治宫颈癌及癌前病变的关键。 目的：利用实时荧光定量PCR法检测高危型人乳头瘤病毒DNA，结合病理诊断分析子宫颈病变与高危型人乳头瘤病毒感染情况，为子宫颈癌与宫颈上皮内瘤变的早期筛查提供一定的数据参考。 方法：以青海大学附属医院妇产科2011年6月—2012年10月63例患子宫疾病的妇女作为研究对象，采用实时荧光定量PCR法检测63例子宫颈疾病患者宫颈分泌物中的高危型人乳头瘤病毒DNA，对检测结果进行收集、整理，结合病理诊断分析子宫颈病变与高危型人乳头瘤病毒感染情况。 结果：采用实时荧光定量PCR法检测63例子宫颈分泌物中的HR-HPV DNA，结果显示血浆HPV-16DNA检出率为：宫颈炎11.1%、CINⅡ期20%、CINⅢ期20%、宫颈癌不同分期检出率为：33.3%、58.3%、75%；血浆HPV-18阳性数结果分别为：宫颈炎16.7%、CINⅡ期16.6%、CINⅢ期10%、宫颈癌不同分期检出率为：15%、16.6%、16.6%。统计学分析提示宫颈癌临床分期与血浆病毒检出率显著相关(P0.05) 结论：实时荧光定量PCR法检测血液中人乳头瘤病毒DNA是比较灵敏的，，用这种方法可以在宫颈癌的筛查中起到一定的作用。子宫颈病变的发生、发展与HR-HPV感染有关，随子宫颈病变的加重，HR-HPV感染阳性率明显升高。
[Abstract]:Cervical cancer is one of the most common malignant tumors in the female reproductive system, which is a global public health problem. In 2008, there were about 529800 new cervical cancer cases and 255100 deaths in the world, of which 85% of the new cases were in developing countries. It is believed that cervical cancer is developed from cervical intraepithelial neoplasias cin, and the occurrence and development of cervical cancer is the result of multiple risk factors. High-risk human papillomavirusHR-HPVs through sexual transmission are one of the high risk factors for cervical cancer and its precancerous lesions. HR-HPV infection can be prevented and cured by medical intervention. Early detection of HR-HPV infection and active intervention are the key to prevent and treat cervical cancer and precancerous lesions. Objective: to detect the high risk human papillomavirus (HPVV) DNA by real-time fluorescence quantitative PCR, and to analyze the cervical lesions and human papillomavirus (HPVV) infection in combination with pathological diagnosis. To provide some data for early screening of cervical cancer and cervical intraepithelial neoplasia. Methods: from June 2011 to October 2012, 63 women with uterine diseases were enrolled in the study of gynecology and obstetrics in the affiliated Hospital of Qinghai University. High-risk human papillomavirus DNA in cervical secretions of 63 patients with cervical diseases was detected by real-time fluorescent quantitative PCR, and the results were collected and sorted out. Combined with pathological diagnosis, cervical lesions and high-risk human papillomavirus infection were analyzed. Results: HR-HPV DNA was detected by real-time fluorescence quantitative PCR in 63 cases of cervical secretion. The results showed that the detection rate of HPV-16 DNA in plasma was: cervicitis 11.1and cin 鈪

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