吉西他滨对卵巢癌移植瘤小鼠的免疫调节作用

发布时间：2018-06-16 10:22

本文选题：吉西他滨 + 卵巢癌　；参考：《中国肿瘤生物治疗杂志》2017年10期

【摘要】：目的:观察吉西他滨(gemcitabine,GEM)对卵巢癌移植瘤小鼠肿瘤免疫微环境的调节作用。方法:C57BL/6小鼠皮下注射卵巢癌ID8细胞构建卵巢癌移植瘤模型,实验组腹腔注射GEM,对照组注射生理盐水,观察肿瘤生长情况。流式细胞术检测小鼠脾脏及肿瘤组织的调节性T细胞(regulatory T cells,Treg)及髓来源抑制性细胞(myeloid-derived suppressor cell,MDSC),实时定量PCR检测小鼠肿瘤组织arginase-1(Arg-1)和Foxp3 mRNA的表达,流式细胞仪检测小鼠脾脏CD8+T细胞的比例,ELLSE法检测小鼠血清IFN-γ及IL-2的表达。结果:GEM处理后的移植瘤小鼠肿瘤生长明显受到抑制[(366.8±44.88)vs(499.3±24.14)mm~3,P0.01]。移植瘤小鼠肿瘤组织及脾脏Treg比率明显降低[(12.71±2.31)%vs(20.36±2.65)%、(10.09±1.69)%vs(13.79±1.31)%,均P0.01]。实验组的相关基因m RNA表达较对照组显著降低[Foxp3:(4.30±0.46)vs(6.35±0.58);Arg-1:(16.32±0.38)vs(13.26±0.37);均P0.01]。实验组血清中的IFN-γ和IL-2含量明显高于对照组[IFN-γ:(71.90±2.28)vs(53.91±3.91)pg/ml;IL-2:(51.46±1.69)vs(40.90±1.50)pg/ml,均P0.01]。结论:GEM下调卵巢癌移植瘤小鼠免疫抑制活性,并可上调小鼠抗肿瘤免疫原性,该结果可为GEM作为卵巢癌免疫治疗干预措施提供实验依据。
[Abstract]:Aim: to investigate the regulatory effect of gemcitine gel on tumor immune microenvironment in mice with ovarian cancer transplanted tumor. Methods C57BL / 6 mice were injected with ovarian cancer ID8 cells subcutaneously to establish ovarian cancer transplanted tumor model. The experimental group was injected with GEM intraperitoneally and the control group was injected with normal saline to observe the tumor growth. The expression of arginase-1 Arg-1 (Arg-1) and Foxp3 mRNA were detected by flow cytometry in mouse spleen and tumor tissues. The proportion of CD8 T cells in spleen of mice was detected by flow cytometry and the expression of IFN- 纬 and IL-2 in serum of mice was detected by ELLSE method. Results the tumor growth was significantly inhibited in the transplanted tumor mice treated with 10% gem [366.8 卤24.14mm ~ (3) 44.88)vs(499.3 卤24.14mm ~ (3 +) P _ (0.01)]. The ratio of Treg in tumor tissue and spleen was significantly decreased in transplanted tumor mice [12.71 卤2.31)%vs(20.36 卤2.65]. The mRNA expression of related genes in the experimental group was significantly lower than that in the control group [Foxp3: 1 + Arg-1 + Arg-1]. All of them were significantly lower than that of the control group (P0.01). The serum levels of IFN- 纬 and IL-2 in the experimental group were significantly higher than those in the control group [IFN- 纬: 71.90 卤3.91 2.28)vs(53.91 卤3.91 渭 g / ml > 51.46 卤1.69)vs(40.90 卤1.50 PG / ml, respectively (P0.01). Conclusion: Genem can down-regulate the immunosuppressive activity and up-regulate the anti-tumor immunogenicity of mice with ovarian cancer transplantation, which may provide experimental evidence for gem as an intervention measure for ovarian cancer immunotherapy.
【作者单位】：河北医科大学第二医院肿瘤科;石家庄市人民医院妇科;河北省人民医院普外科&肿瘤科;
【分类号】：R737.31

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