MiR-26b在子痫前期胎盘中差异表达的临床意义

发布时间：2018-06-16 11:24

本文选题：MiR-b + 子痫前期　；参考：《中国妇产科临床杂志》2017年03期

【摘要】：目的探讨子痫前期胎盘组织中miR-26b差异表达及其临床意义。方法收集子痫患者与健康人胎盘组织各30例,RT-qPCR和Western blotting检测组织中miR-26b与Smad1的表达;转染miR-26b的mimics到HTR-8/SVneo细胞中,MTT法比较转染组、NC对照组细胞增殖情况,流式细胞术检测各组细胞周期情况;同时RT-qPCR、Western blotting检测各组miR-26b和Smad1的表达情况,并运用双荧光素酶报告检测试验验证miR-26b与靶标Smad1之间靶向关系。结果 miR-26b在子痫前期胎盘组织中高表达,且Smad1的表达与miR-26b的表达相反;滋养层细胞中miR-26b过表达会抑制细胞增殖(P0.01),并将细胞阻滞在G0/G1期。miR-26b过表达会降低Smad1 mRNA与蛋白的表达(P0.01)。双荧光素酶报告基因实验证明Smad1是miR-26b的直接靶点。结论 miR-26b可能通过靶向Smad1调控HTR-8/SVneo细胞的增殖与周期。
[Abstract]:Objective to investigate the differential expression of miR-26b in placenta of preeclampsia and its clinical significance. Methods the expression of miR-26b and Smad1 in 30 cases of placental tissues from patients with eclampsia and 30 cases of healthy subjects were detected by RT-PCR and Western blotting, and the proliferation of NC cells in the transfected group was compared by mimics transfected with miR-26b into HTR-8 / SVneo cells. The expression of miR-26b and Smad1 in each group was detected by RT-qPCRG Western blotting, and the targeting relationship between miR-26b and target Smad1 was verified by double-luciferase report assay. Results the expression of miR-26b in placenta of preeclampsia was higher than that of miR-26b, and the overexpression of miR-26b in trophoblast cells inhibited the proliferation of P0.01and blocked the cells in G0 / G1 phase. MiR-26b overexpression decreased the expression of Smad1 mRNA and protein. Double luciferase reporter gene experiment showed that Smad1 was a direct target of miR-26b. Conclusion miR-26b may regulate the proliferation and cell cycle of HTR-8 / SVneo cells by targeting Smad1.
【作者单位】：郑州市第二人民医院妇产科;郑州大学第五附属医院妇产科;
【分类号】：R714.244

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