宫腔粘连模型大鼠子宫内膜胞饮突发育和整合素β3表达

发布时间：2018-06-21 04:33

本文选题：宫腔粘连 + 内膜容受性　；参考：《现代妇产科进展》2017年05期

【摘要】：目的:建立宫腔粘连大鼠模型,观察子宫内膜胞饮突的发育和整合素β3的表达,探讨宫腔粘连对子宫内膜容受性的影响。方法:选取健康SD大鼠30只,将每只大鼠左侧子宫作为模型组(宫腔注入95%无水乙醇,持续5min),右侧子宫作为对照组(注入等量生理盐水)。术后两个动情周期取子宫,光镜观察子宫内膜形态学变化及纤维化程度,扫描电镜观察大鼠子宫内膜胞饮突的发育情况,免疫组化法检测子宫内膜整合素β3表达。结果:模型组大鼠子宫内膜明显变薄,上皮细胞排列紊乱,间质内胶原纤维增多,子宫内膜纤维化评分为(4.69±0.22)分,明显高于对照组。扫描电镜观察,对照组大鼠子宫内膜可见大量发育完全的胞饮突,而模型组子宫内膜胞饮突分布稀少,表面皱缩,发育不同步。模型组的整合素β3表达明显低于对照组,差异有统计学意义(P0.05)。结论:注射无水乙醇可建立稳定的大鼠宫腔粘连动物模型,抑制子宫内膜胞饮突和整合素β3表达,降低子宫内膜的容受性。
[Abstract]:Aim: to establish a rat model of intrauterine adhesion, to observe the development of endometrial cell processes and the expression of integrin 尾 3, and to explore the effect of uterine adhesion on endometrial receptivity. Methods: 30 healthy SD rats were selected. The left uterus of each rat was used as the model group (95% anhydrous alcohol was injected into the uterine cavity for 5 min) and the right uterus was used as the control group (injected with the same amount of normal saline). The changes of endometrium morphology and the degree of fibrosis were observed by light microscope, the development of the ectopic process of endometrium was observed by scanning electron microscope, and the expression of integrin 尾 3 in endometrium was detected by immunohistochemical method. Results: in the model group, the endometrium became thinner, the epithelial cells arranged disorderly, the collagen fibers in the interstitial increased, and the endometrial fibrosis score was 4.69 卤0.22), which was significantly higher than that in the control group. Scanning electron microscopy (SEM) showed that a large number of fully developed ectopic processes were observed in the endometrium of the control group, while in the model group, the distribution of the ectopic process was rare, the surface of the endometrium shrank, and the development of the endometrium was not synchronous. The expression of integrin 尾 3 in the model group was significantly lower than that in the control group (P 0.05). Conclusion: injection of anhydrous ethanol can establish a stable rat model of intrauterine adhesion, inhibit the expression of ectopic and integrin 尾 3 in endometrium, and decrease the receptivity of endometrium.
【作者单位】：河北省人民医院妇一科;解放军白求恩国际和平医院药剂科;
【基金】：2015年度河北省医学科学研究重点课题计划(No:20150122)
【分类号】：R-332;R711.74

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