超低温冷冻对人精子糖被的影响

发布时间：2018-06-21 05:11

本文选题：精子 + 糖被　；参考：《中华生殖与避孕杂志》2017年04期

【摘要】：目的研究超低温冷冻对人精子糖被的损伤。方法利用凝集素芯片比较精子冷冻前、后的凝集素结合谱。结果通过比较冷冻保护剂和冷冻方法对精子复苏率的影响,确定了Cryo Sperm TM以及一步熏蒸法为最佳精子冷冻试剂和方法;通过凝集素芯片比较精子冷冻前、后的凝集素结合谱观察到在91种凝集素中,有33种发生了显著变化,其中,冷冻后显著下调的有9种,显著上调的有24种,并且显著下调的MAA及显著上调的PSA、ABA和AIA经流式验证,结果与芯片一致。结论精子糖被经过冷冻之后发生了显著变化,对精子起保护作用的唾液酸大量丢失,同时也暴露糖链内部的糖基,因此精子糖被的冷冻损伤可能是由于低温冷冻引起精子生育力低下的原因之一。
[Abstract]:Objective to study the effect of cryopreservation on the sucrose of human spermatozoa. Methods Lectin binding spectra before and after cryopreservation were compared by agglutinin chip. Results Cryo Sperm TM and one-step fumigation were determined as the best resuscitation reagent and method by comparing the effects of cryopreservation agents and freezing methods on sperm resuscitation rate. Among the 91 kinds of lectins, 33 had significant changes, among which 9 were significantly down-regulated, 24 were up-regulated, and the down-regulated MAA and PSA-ABA and AIA were significantly up-regulated by flow cytometry. The result is consistent with the chip. Conclusion there is a significant change in sperm sugar after freezing. The sialic acid that protects spermatozoa is largely lost, and the glycosyl group inside the sugar chain is also exposed. Therefore, the cryopreservation injury of sperm sugar may be one of the reasons of low sperm fertility caused by cryopreservation.
【作者单位】：华东理工大学生物反应器工程国家重点实验室;复旦大学附属妇产科医院上海集爱遗传与不育诊疗中心;上海交通大学医学院附属仁济医院过敏科;国家人口和计划生育委员会计划生育药具重点实验室上海市计划生育科学研究所;上海交通大学系统生物医学研究院;上海交通大学附属第一人民医院男科上海交通大学泌尿外科研究所男性中心;中国科学院上海生命科学研究院生物化学与细胞生物学研究所;
【基金】：国家青年科学基金项目(81401252) 默克雪兰诺中国生殖医学研究基金~~
【分类号】：R714.8

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