Nanog介导FSH诱导卵巢癌转移和侵袭的研究

发布时间：2018-07-01 10:57

本文选题：卵巢癌 + 卵泡刺激素　；参考：《现代妇产科进展》2015年05期

【摘要】：目的:探讨Nanog在FSH诱导的卵巢癌转移和侵袭中的作用。方法:采用Western blot法检测永生化卵巢上皮细胞Moody,良性卵巢肿瘤细胞Mcv152和卵巢癌细胞Hey、Ho8910、SKOV3中Nanog表达。使用FSH以浓度梯度和时间梯度的方式处理SKOV3细胞,Western blot法检测Nanog蛋白表达。使用FSH处理si-Con或si-Nanog转染卵巢癌SKOV3细胞,实验分组:0.1%DMSO+转染空白干扰RNA组(Con+si-Con)、FSH+转染空白干扰RNA组(FSH+si-Con)、0.1%DMSO+转染Nanog干扰RNA组(Con+siNanog)和FSH+转染Nanog干扰RNA组(FSH+si-Nanog)。使用划痕实验和Transwell侵袭实验检测4组细胞的转移和侵袭能力,Western blot法分析4组细胞中Nanog蛋白表达。应用免疫组化法检测卵巢癌组织中Nanog蛋白表达情况。结果:卵巢癌细胞系Hey、Ho8910、SKOV3中Nanog表达明显高于永生化和良性卵巢上皮细胞系(P0.05)。FSH上调Nanog表达以浓度和时间依赖的方式。对照组(Con+si-Con,Con+si-Nanog)的迁移和侵袭能力明显低于实验组(FSH+si-Con,FSH+si-Nanog)(P0.05)。卵巢癌组织中Nanog表达水平明显高于正常卵巢组织(P0.05),Nanog蛋白表达与卵巢癌转移相关。结论:Nanog是FSH重要的下游靶基因,介导FSH诱导的卵巢癌转移作用。
[Abstract]:Objective: to investigate the role of Nanog in FSH induced metastasis and invasion of ovarian cancer. Methods: the expression of Nanog in immortalized ovarian epithelial cell, benign ovarian tumor cell Mcv152 and ovarian cancer cell Heyhiho8910SKOV3 was detected by Western blot assay. SKOV3 cells were treated with FSH as concentration gradient and time gradient to detect the expression of Nanog protein by Western blot. SKOV3 cells transfected with si-Con or si-Nanog were treated with FSH. The SKOV3 cells were transfected with FSH si-Con (Con si-Con) and FSH si-Con (FSH si-Con) with FMSO (0.1 DMSO) and FSH transfected Nanog interference RNA (Con siNanog) and FSH transfected Nanog interference RNA (FSH si-Nanog) respectively. The expression of Nanog protein was detected by Western blot assay. The expression of Nanog protein in ovarian cancer tissues was detected by immunohistochemical method. Results: compared with immortalized and benign ovarian epithelial cell line (P0.05), the expression of Nanog in ovarian cancer cell line HeyangHo8910SKOV3 was significantly higher than that in benign ovarian epithelial cell line (P0.05). FSH upregulated Nanog expression in a dose-and time-dependent manner. The migration and invasion ability of the control group (Con si-Cono Con si-Nanog) was significantly lower than that of the experimental group (FSH si-Conn FSH si-Nanog) (P0.05). The expression of Nanog protein in ovarian cancer tissues was significantly higher than that in normal ovarian tissues (P0.05). ConclusionWhile Nanog is an important downstream target gene of FSH, it mediates the metastasis of ovarian cancer induced by FSH.
【作者单位】：上海交通大学附属上海市第一人民医院妇产科;上海市嘉定区妇幼保健院;
【基金】：国家自然科学基金项目(No:81172478)
【分类号】：R737.31

【共引文献】