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E7蛋白在HPV16变异体持续感染患者宫颈组织中的表达

发布时间:2018-07-16 15:38
【摘要】:目的:探索HPV16亚洲变异体E6 T178G及欧洲变异体E6 T350G,A442C与其他变异体致宫颈病变及机制的异同。方法:选取浦东新区人民医院门诊或住院患者中HPV16型单一阳性、宫颈细胞学正常的300例研究对象,予宫颈液基薄层细胞学检查(Thinprep cytologic test,TCT)采集宫颈脱落细胞,对其E2、E6、E7基因序列进行检测;1年后复查TCT、HPV,持续感染者再次基因测序明确HPV16变异体分型,并对持续感染者进行阴道镜活检后病理检查,对所有患者均用SP免疫组化法检测E7蛋白表达水平。结果:HPV16变异体持续感染1年后,292例成功测序的患者中,259例慢性宫颈炎,32例宫颈上皮内瘤变Ⅰ级(CINI),1例宫颈上皮内瘤变Ⅰ级(CINⅡ)。E7蛋白在各种变异体感染的患者宫颈组织中均有表达,其在亚洲变异体及欧洲变异体感染组与其他变异体感染组之间表达无明显差异(P0.05)。结论:E7蛋白可能在HPV16变异体致宫颈病变的早期阶段即发挥作用,但E7蛋白可能并不是HPV16不同变异体致宫颈病变机制不同的参考指标。
[Abstract]:Objective: to explore the similarities and differences of cervical lesions caused by HPV16 Asian variant E6T178G and European variant E6T350GA442C with other variants. Methods: cervical exfoliated cells were collected from 300 patients with single HPV16 positive and normal cervical cytology in the outpatient or inpatient of Pudong New area people's Hospital. The cervical exfoliated cells were collected by Thinprep cytologic test TCT (Thinprep cytologic test TCT). The sequence of E2OE6 E7 gene was detected, and a year later, the HPV16 variant was determined by resequencing of the HPV16 gene, and the colposcopy biopsy was performed on the HPV16 mutation in the patients with persistent infection after colposcopy biopsy, and the HPV16 mutation was determined by re-sequencing of the HPV16 gene. The expression of E7 protein was detected by SP immunohistochemical method in all patients. Results one year after the persistent infection of the 10% HPV16 variant, 292 patients were successfully sequenced in 259 cases of chronic cervicitis 32 cases of cervical intraepithelial neoplasia grade I (CINI) and 1 case of cervical intraepithelial neoplasia grade 鈪,

本文编号:2126843

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