宫颈鳞癌组织中β-catenin、E-cadherin、PTEN蛋白表达变化及其意义

发布时间：2018-07-30 08:40

【摘要】：目的观察宫颈鳞癌组织中β-连环蛋白(β-catenin)、E-钙黏蛋白(E-cadherin)、磷酸酶及张力蛋白同源(PTEN)蛋白的表达变化,并探讨其临床意义。方法 82例份宫颈鳞癌组织标本为观察组,32例份宫颈上皮内瘤变(CIN)组织标本为CIN组,30例份正常宫颈组织标本为正常组。采用免疫组化SP法进行检测各组β-catenin、Ecadherin及PTEN蛋白,表达与宫颈癌临床病理参数的关系,分析β-catenin、E-cadherin及PTEN蛋白的相关性,采用ROC曲线判断β-catenin、E-cadherin及PTEN蛋白在宫颈癌诊断中的灵敏度及特异度。结果观察组β-catenin阳性表达率高于CIN组和对照组,E-cadherin、PTEN蛋白阳性表达率均低于CIN组和对照组(P均0.05)。CIN组β-catenin阳性表达率高于对照组,E-cadherin、PTEN蛋白阳性表达率均低于对照组(P均0.05)。β-catenin表达与宫颈鳞癌组织学分级、临床分期及淋巴结转移相关(P均0.05);E-cadherin、PTEN蛋白表达与宫颈鳞癌组织学分级、临床分期、浸润深度与淋巴结转移相关(P均0.05)。β-catenin诊断宫颈鳞癌的灵敏度、特异度分别为89.02%、87.10%,E-cadherin诊断宫颈鳞癌的灵敏度、特异度分别为85.37%、83.87%,PTEN蛋白诊断宫颈鳞癌的灵敏度、特异度分别为84.15%、85.48%。宫颈鳞癌组织中β-catenin表达与E-cadherin、PTEN呈负相关(r分别为-0.736,-0.702;P0.05),E-cadherin表达与PTEN蛋白表达呈正相关(r=0.694,P0.05)。结论宫颈鳞癌组织中β-catenin高表达,E-cadherin、PTEN蛋白低表达。β-catenin、E-cadherin及PTEN蛋白诊断宫颈鳞癌的敏感度和特异度均较高。β-catenin、E-cadherin及PTEN在宫颈鳞癌的发生发展中可能存在相互作用。
[Abstract]:Objective to observe the expression of 尾 -catenin (尾 -catenin) E-cadherin (E-cadherin), phosphatase and tensin homologous (PTEN) protein in cervical squamous cell carcinoma (SCC) and to explore its clinical significance. Methods Eighty-two specimens of cervical squamous cell carcinoma were collected from 32 patients with cervical intraepithelial neoplasia (CIN) and 30 normal cervix tissues from CIN group. Immunohistochemical SP method was used to detect the expression of 尾 -cateninine E-cadherin and PTEN protein, and the relationship between the expression of 尾 -cateninine E-cadherin and PTEN protein was analyzed. The sensitivity and specificity of 尾 -cateninine E-cadherin and PTEN protein in the diagnosis of cervical cancer were evaluated by ROC curve. Results the positive expression rate of 尾 -catenin in observation group was higher than that in CIN group and control group. The positive expression rate of 尾 -catenin protein in observation group was lower than that in CIN group and control group (P 0.05). The positive expression rate of 尾 -catenin protein in cin group was higher than that in control group. And histological grade of cervical squamous cell carcinoma, The expression of E-cadherin PTEN protein was correlated with the histological grade, clinical stage, depth of invasion and lymph node metastasis (P 0.05). The sensitivity of 尾 -catenin in the diagnosis of cervical squamous cell carcinoma was significantly correlated with the clinical stage and lymph node metastasis (P 0.05). The sensitivity of E-cadherin in the diagnosis of cervical squamous cell carcinoma was 89.02 and 87.10, and the specificity was 85.37 and 83.87, respectively. The specificity of PTEN protein in the diagnosis of cervical squamous cell carcinoma was 84.15 and 85.48, respectively. There was a negative correlation between the expression of 尾 -catenin and E-cadherin in cervical squamous cell carcinoma (r = -0.736- 0.702P respectively). The expression of E-cadherin was positively correlated with the expression of PTEN protein (r = 0.694P 0.05). Conclusion the high expression of 尾 -catenin and the low expression of E-cadherin PTEN protein in cervical squamous cell carcinoma. The sensitivity and specificity of 尾 -cateninine E-cadherin and PTEN protein in the diagnosis of cervical squamous cell carcinoma are higher. There may be interaction between 尾 -catenin E-cadherin and PTEN in the occurrence and development of cervical squamous cell carcinoma.
【作者单位】：华中科技大学同济医学院附属普爱医院;
【基金】：湖北省自然科学基金资助项目(2014CFB36)
【分类号】：R737.33

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