肿瘤坏死因子-α基因多态性与妊娠期糖尿病患者及子代关系的实验性研究

发布时间：2018-07-31 05:10

【摘要】：目的：探讨肿瘤坏死因子α基因-857C/T、-863A/C和-1031T/C三个位点单核苷酸多态性与妊娠期糖尿病的关系以及子代（胎儿）与母亲之间的关系。方法：随机选取2012年3月至2013年2月在北京航天总医院妇产科正规产检的足月妊娠孕妇100例，将其分为妊娠期糖尿病组（GDM组）和正常孕妇（对照组）组，每组各50例，抽取孕妇外周静脉血及其新生儿脐带血，提取DNA，采用PCR限制性酶切片段长度分析-857C/T、-863A/C和-1031T/C，测序验证。应用2检验比较基因型分布是否符合Hardy-Weinberg平衡。结果采用t检验和2检验等统计学分析。结果：TNF-α基因-857C/T位点的基因型分布在GDM组与正常孕妇组有显著差异，，其中-857位点在GDM组中CT基因型及T等位基因频率所占比例高于正常孕妇组， P0.05；另外，在基因型为CC的母亲中，GDM组胎儿CT型比例高于正常对照组胎儿， P0.05。而-1031C/T和-863A/C位点无论是基因型还是等位基因频率在两组母亲中均无差异，P0.05。三个位点的基因型分布在其胎儿中均有显著差异，其中-857C/T位点在GDM组胎儿中CT基因型及T等位基因频率所占比例高于正常孕妇组胎儿，P0.05。-863A/C位点在GDM组胎儿中CA基因型及A等位基因频率所占比例高于正常孕妇组胎儿， P 0.05。-1031T/C位点在GDM组胎儿中CT基因型及C等位基因频率所占比例高于正常孕妇组胎儿,P0.05。结论：1.TNF-α基因-857C/T等位基因多态性与GDM的发病有关；-863A/C和-1031C/T两个位点可能与GDM无关。 2.胎儿的TNF-α基因3种SNP可增加母亲患GDM风险。
[Abstract]:Aim: to investigate the relationship between the single nucleotide polymorphisms (SNP) at three loci of tumor necrosis factor 伪 gene -857C- 863A / C and -1031T / C and gestational diabetes mellitus (GDM), as well as the relationship between the offspring (fetus) and the mother. Methods: from March 2012 to February 2013, 100 pregnant women in Beijing Aerospace General Hospital were randomly divided into gestational diabetes mellitus (GDM) group and normal pregnant women (control group) group, with 50 pregnant women in each group. Peripheral venous blood of pregnant women and umbilical cord blood of newborns were extracted. PCR restriction fragment length analysis was used to analyze -857C / T- 863A / C and -1031T / C respectively. The genotype distribution was compared with Hardy-Weinberg equilibrium by test 2. Results t test and 2 test were used for statistical analysis. Results there was significant difference in the genotype distribution of -857C / T locus between GDM group and normal pregnant woman group, and the proportion of CT genotype and T allele frequency in GDM group was higher than that in normal pregnant woman group (P0.05). The proportion of fetal CT type in GDM group was higher than that in normal control group (P0.05). The frequencies of genotype and allele at -1031 C / T and -863 A / C loci were not significantly different between the two groups (P 0.05). The genotypes of the three loci were significantly different in their fetuses. The frequency of CT genotype and T allele in GDM group was higher than that in normal pregnant woman group (P 0.05.-863 A / C locus). CA genotype and A allele frequency in GDM group was higher than that in normal pregnant woman group. The percentage of CT genotype and C allele frequency in GDM group was higher than that in normal pregnant women group (P 0.05). Conclusion: 1. TNF- 伪 gene -857C / T allele polymorphism is associated with the pathogenesis of GDM. The two loci of -863 A / C and -1031 C / T may not be associated with GDM. 2. Fetal TNF- 伪 gene three types of SNP may increase the maternal risk of GDM.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.256

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