TGF-β1调节miR-99家族对卵巢癌细胞恶性行为的影响

发布时间：2018-08-12 17:41

【摘要】：目的:探讨TGF-β1通过调节miR-99家族各miRNA对卵巢癌细胞增殖、迁移及侵袭行为的影响。方法:TGF-β1、miR-99家族各miRNA模拟物、miR-99家族各miRNA抑制物分别刺激或转染卵巢癌细胞系A2780及SKOV3。实时荧光定量PCR检测miR-99家族各miRNA表达水平。CCK-8法检测细胞增殖能力。划痕实验检测细胞迁移能力。Transwell检测细胞侵袭及迁移能力。结果:与空白对照组比较,TGF-β1处理组卵巢癌细胞中miR-99家族各miRNA表达上调(P0.01),卵巢癌细胞增殖、迁移及侵袭能力明显增强(P0.05);miR-99家族各miRNA模拟物转染组细胞增殖减弱(P0.05),迁移及侵袭能力增强(P0.05);miR-99家族各miRNA抑制物转染组细胞增殖增强(P0.05),迁移及侵袭能力减弱(P0.05)。结论:TGF-β1可通过调节miR-99家族表达水平促进卵巢癌细胞系A2780及SKOV3的增殖、迁移及侵袭能力,TGF-β1及miR-99家族表达水平可能作为卵巢癌恶性程度预测及预后判断的参考指标。
[Abstract]:Aim: to investigate the effects of TGF- 尾 1 on the proliferation, migration and invasion of ovarian cancer cells by regulating the miRNA of miR-99 family. Methods the miRNA mimics of TGF- 尾 1 and miR-99 were stimulated or transfected into ovarian cancer cell lines A2780 and SKOV3, respectively. Real-time fluorescence quantitative PCR was used to detect the miRNA expression of miR-99 family. CCK-8 method was used to detect cell proliferation. The ability of cell migration was detected by scratch assay. The ability of cell invasion and migration was detected by Transwell. Results: compared with the control group, the miRNA expression of miR-99 family was up-regulated (P0.01), and the proliferation of ovarian cancer cells was observed in TGF- 尾 1 treated group. The ability of migration and invasion was significantly increased (P0.05). (P0.05) the cell proliferation was decreased (P0.05), the migration and invasion ability was increased (P0.05), and the migration and invasion ability was weakened (P0.05) in the miRNA inhibitor transfection group of the MiR-99 family (P0.05). Conclusion the expression of TGF- 尾 1 can promote the proliferation of ovarian cancer cell line A2780 and SKOV3 by regulating the expression level of miR-99 family. The expression of TGF- 尾 1 and miR-99 family in ovarian cancer cell line A2780 and SKOV3 may be used as a reference index for predicting the malignant degree and judging prognosis of ovarian cancer.
【作者单位】：青岛大学附属医院妇科;青岛大学;青岛市第八人民医院妇科;
【基金】：国家自然科学基金资助项目(No:81100697) 山东省博士基金资助项目(No:BS2001SW003)
【分类号】：R737.31

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