新鲜周期和复苏周期囊胚培养及移植的临床结局分析

发布时间：2018-10-15 21:08

【摘要】：目的:比较新鲜周期和复苏周期囊胚培养及移植的临床结局,探讨囊胚移植的最佳方案。方法:回顾分析2012年1月至2014年8月在郑州大学第三附属医院生殖中心行常规体外受精/卵胞质内单精子注射-胚胎移植(IVF/ICSI-ET)患者的临床资料。根据培养方式分为A组(新鲜胚胎行囊胚培养后移植,58个周期)、B组(新鲜胚胎行囊胚培养冷冻复苏后移植,285个周期)和C组(冷冻胚胎复苏后行囊胚培养再移植,84个周期)。比较新鲜胚胎和冷冻胚胎复苏后的囊胚形成率及可利用囊胚形成率以及3组患者的囊胚种植率、生化妊娠率、临床妊娠率、多胎率和早期流产率等指标。结果:新鲜胚胎的囊胚形成率及可利用囊胚形成率均高于冷冻胚胎(χ2=8.561和4.037,P0.05);3组患者的生化妊娠率、临床妊娠率及早期流产率比较差异有统计学意义(χ2=44.376、6.255和9.167,P0.05),B组患者的生化妊娠率及临床妊娠率均高于A组,早期流产率低于A组;3组间囊胚种植率和多胎率比较差异无统计学意义(χ2=4.234、1.172,P0.05)。结论:新鲜胚胎较冷冻胚胎有较高的囊胚形成率,新鲜囊胚冷冻后择期复苏移植能提高患者的妊娠率,是目前最佳的囊胚培养和移植方案。
[Abstract]:Objective: to compare the clinical outcome of blastocyst culture and transplantation in fresh cycle and resuscitation cycle, and to explore the best scheme of blastocyst transplantation. Methods: the clinical data of patients undergoing in vitro fertilization (IVF) / intracytoplasmic sperm injection (IVF/ICSI-ET) were retrospectively analyzed from January 2012 to August 2014 in the Reproductive Center of the third affiliated Hospital of Zhengzhou University. According to the culture method, group A (fresh embryos were transferred after blastocyst culture, 58 cycles), B group (fresh embryos were transferred after cryopreservation, 285 cycles) and group C (frozen embryo resuscitation after blastocyst culture and retransplantation, 84 cycles). The rates of blastocyst formation, blastocyst formation and blastocyst implantation rate, biochemical pregnancy rate, clinical pregnancy rate, multiple pregnancy rate and early abortion rate were compared between fresh and frozen embryos. Results: the blastocyst formation rate and the usable blastocyst formation rate of fresh embryos were higher than those of frozen embryos (蠂 2 8.561 and 4.037 P 0.05). There was significant difference in clinical pregnancy rate and early abortion rate (蠂 ~ 2 / 44.3766.255 and 9.167g /), B, respectively). The biochemical pregnancy rate and clinical pregnancy rate were higher in group A than in group A, and the rate of early abortion was lower than that in group A, and there was no significant difference among the three groups in blastocyst implantation rate and multiple pregnancy rate (蠂 ~ (24.2341.172P). Conclusion: fresh embryos have higher blastocyst formation rate than frozen embryos. Selective resuscitation of fresh blastocysts after cryopreservation can improve the pregnancy rate of patients and is the best way to culture and transfer blastocysts.
【作者单位】：郑州大学第三附属医院生殖中心;
【分类号】：R714.8

【共引文献】