Cx43基因转染对人卵巢癌SKOV3细胞增殖和迁移能力的影响

发布时间：2018-11-09 18:01

【摘要】：目的观察缝隙连接蛋白43(Cx43)基因修饰对人卵巢癌SKOV3细胞增殖和迁移能力的影响。方法SKOV3细胞分为观察组、对照组和空白组,观察组和对照组分别用重组表达质粒p Bud CE4.1-Cx43、空载质粒p Bud CE4.1转染,空白组不做任何处理。分别采用RT-PCR法、Western blot法检测各组Cx43 mRNA及蛋白表达,采用划痕荷载染料传输实验检测细胞间通讯功能,采用流式细胞仪观察细胞周期的变化,采用CCK-8法、划痕愈合实验、细胞侵袭实验分别观察细胞的增殖、迁移及侵袭能力的变化。结果与对照组和空白组相比,观察组Cx43mRNA及蛋白表达均明显升高,细胞传递的荧光强度明显增高,细胞周期处于G0/G1期数目较多,S期的细胞数目明显降低;细胞增殖降低,迁移和侵袭能力均明显下降,P均0.05。结论 Cx43可抑制SKOV3细胞的增殖和迁移,可作为卵巢癌治疗的新靶点。
[Abstract]:Objective to investigate the effect of gap junction protein 43 (Cx43) gene modification on proliferation and migration of human ovarian cancer SKOV3 cells. Methods SKOV3 cells were divided into the observation group, the control group and the blank group. The observation group and the control group were transfected with the recombinant expression plasmid p Bud CE4.1-Cx43, empty plasmid p Bud CE4.1, and the blank group was not treated with any treatment. The expression of Cx43 mRNA and protein in each group was detected by RT-PCR, Western blot method, the intercellular communication function was detected by scratch load dye transfer test, the cell cycle was observed by flow cytometry, and the scratch healing test was performed by CCK-8 method. Cell invasion assay was used to observe the changes of cell proliferation, migration and invasion ability. Results compared with the control group and the blank group, the expression of Cx43mRNA and protein in the observation group was significantly increased, the fluorescence intensity of cell transmission was significantly increased, the number of cell cycle in G0/G1 phase was more, and the number of cells in S phase was significantly decreased. Cell proliferation decreased, migration and invasion decreased significantly (P < 0.05). Conclusion Cx43 can inhibit the proliferation and migration of SKOV3 cells and be a new target for ovarian cancer therapy.
【作者单位】：静海县医院;天津医科大学总医院;
【分类号】：R737.31

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