子宫内膜腺癌组织中CD68、YKL-40表达及其相关性研究
发布时间：2018-11-10 11:12
【摘要】：目的： 分析子宫内膜腺癌组织中CD68和YKL-40蛋白的表达水平，探讨二者与子宫内膜癌临床病理特征之间的关系及其临床意义。方法： 1.采用免疫组织化学Elivision方法检测CD68和YKL-40蛋白分别在20例正常子宫内膜（normal endometrium，NE）、20例子宫内膜不典型增生（endometrialatypical hyperplasia，EAH）和56例子宫内膜腺癌（endometrial adenocarcinoma，EAC）中的表达。2.运用SPSS17.0统计软件对所有研究的数据进行统计学分析和处理。 结果： 1.在NE、EAH、EAC三组组织中均可见CD68蛋白的阳性表达。CD68在NE、EAH、EAC组织中的阳性表达率分别为30%、55%、80.4%。EAC组织中CD68蛋白的阳性表达率明显高于EAH组织（X2=4.887，P=0.0270.05），且也显著高于NE组织（X2=16.929，P=0.0000.05）；EAH组织中CD68蛋白的阳性表达率与NE组织差异无统计学意义（X2=2.558，P=0.110.05）。在EAC组织中CD68蛋白的表达与肿瘤的组织学分级、肌层浸润、及淋巴结转移有关(P=0.019，P=0.047，P=0.005，P均0.05)，而与患者年龄及临床分期无关(P=1.0，P=0.697，P均0.05)。 2.YKL-40蛋白在NE、EAH、EAC三组组织中均可见阳性表达。YKL-40蛋白在NE、EAH、EAC组织中的阳性表达率分别为25%、40%、76.8%。EAC组织中YKL-40蛋白的阳性表达率明显高于EAH组织（X2=9.034， P=0.0030.05），且也同样显著高于NE组织（X2=16.984，P=0.0000.05）；EAH组织中YKL-40蛋白的阳性表达率与NE组织差异无统计学意义（X2=1.026，P=0.3110.05）。在EAC组织中YKL-40蛋白的表达与肿瘤的组织学分级有关(P=0.008，P0.05)，而与患者年龄、临床分期、肌层浸润和淋巴结转移无关(P=0.186，P=0.852，，P=0.511，P=0.752，P均0.05)。 3.经Spearman相关分析显示：CD68与YKL-40蛋白在EAC组织中的表达有相关性，且呈正相关（rs=0.367，P=0.005）。 结论： 1. CD68和YKL-40蛋白在NE、EAH、EAC三组组织中的阳性表达率呈逐步升高的趋势。 2.随着EAC患者的组织分化程度降低、肌层浸润加深和淋巴结转移，CD68蛋白的阳性表达率更高。EAC患者中YKL-40蛋白随组织分化程度降低而阳性表率也更高。 3. CD68和YKL-40蛋白在EAC组织中过表达且呈正相关。
[Abstract]:Objective: to analyze the expression of CD68 and YKL-40 protein in endometrial adenocarcinoma and to explore the relationship between them and the clinicopathological features of endometrial carcinoma. Methods: 1. Immunohistochemical Elivision was used to detect the expression of CD68 and YKL-40 protein in 20 cases of normal endometrium (normal endometrium,NE), 20 cases of endometrial atypical hyperplasia (endometrialatypical hyperplasia,EAH) and 56 cases of endometrial adenocarcinoma (endometrial adenocarcinoma,EAC). The data of all the studies were analyzed and processed by SPSS17.0 software. Results: 1. The positive expression of CD68 protein was found in all three groups of NE,EAH,EAC, and the positive expression rate of CD68 in NE,EAH,EAC tissue was 30% and 55%, respectively. The positive expression rate of CD68 protein in 80.4%.EAC tissues was significantly higher than that in EAH tissues (X2O4.887Pu 0.0270.05), and also higher than that in NE tissues (X2O16.929PU 0.0000.05). The positive expression rate of CD68 protein in EAH was not significantly different from that in NE (X2 + 2.558 P < 0. 110.05). The expression of CD68 protein in EAC was related to histological grade, myometrial infiltration and lymph node metastasis (P0.019, P0.047, P0. 005, P 0. 05), but not to the patient's age and clinical stage (P0. 0. 0 P0. 697, P 0. 697, P 0. 019, P 0. 047, P 0. 005, P 0. 05). P 0.05). Positive expression of 2.YKL-40 protein was found in all three groups of NE,EAH,EAC. The positive expression rates of YKL-40 protein in NE,EAH,EAC tissues were 25% and 40%, respectively. The positive expression rate of YKL-40 protein in 76.8%.EAC was significantly higher than that in EAH (X2 + 9.034, P0. 0030. 05), and was also significantly higher than that in NE (X2 + 16. 984, P0. 0000. 05). The positive expression rate of YKL-40 protein in EAH was not significantly different from that in NE (X _ 2 ~ (2 +) ~ (1.026) P ~ (0.3110.05). The expression of YKL-40 protein in EAC was correlated with the histological grade of the tumor (P0. 008P 0.05), but not with age, clinical stage, myometrial invasion and lymph node metastasis (P0. 186 P0. 852P0. 511 P0. 752, P = 0. 752). P 0.05). 3. Spearman correlation analysis showed that there was a positive correlation (rs=0.367,P=0.005) between CD68 and YKL-40 protein expression in EAC tissues. Conclusion: 1. The positive expression rate of CD68 and YKL-40 protein in three groups of NE,EAH,EAC increased gradually. 2. With the decrease of tissue differentiation, myometrial infiltration and lymph node metastasis, the positive expression rate of CD68 protein was higher in EAC patients, and the positive rate of YKL-40 protein was higher with the decrease of tissue differentiation degree in EAC patients. 3. The overexpression of CD68 and YKL-40 protein in EAC tissues was positively correlated.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33
本文编号：2322311
[Abstract]:Objective: to analyze the expression of CD68 and YKL-40 protein in endometrial adenocarcinoma and to explore the relationship between them and the clinicopathological features of endometrial carcinoma. Methods: 1. Immunohistochemical Elivision was used to detect the expression of CD68 and YKL-40 protein in 20 cases of normal endometrium (normal endometrium,NE), 20 cases of endometrial atypical hyperplasia (endometrialatypical hyperplasia,EAH) and 56 cases of endometrial adenocarcinoma (endometrial adenocarcinoma,EAC). The data of all the studies were analyzed and processed by SPSS17.0 software. Results: 1. The positive expression of CD68 protein was found in all three groups of NE,EAH,EAC, and the positive expression rate of CD68 in NE,EAH,EAC tissue was 30% and 55%, respectively. The positive expression rate of CD68 protein in 80.4%.EAC tissues was significantly higher than that in EAH tissues (X2O4.887Pu 0.0270.05), and also higher than that in NE tissues (X2O16.929PU 0.0000.05). The positive expression rate of CD68 protein in EAH was not significantly different from that in NE (X2 + 2.558 P < 0. 110.05). The expression of CD68 protein in EAC was related to histological grade, myometrial infiltration and lymph node metastasis (P0.019, P0.047, P0. 005, P 0. 05), but not to the patient's age and clinical stage (P0. 0. 0 P0. 697, P 0. 697, P 0. 019, P 0. 047, P 0. 005, P 0. 05). P 0.05). Positive expression of 2.YKL-40 protein was found in all three groups of NE,EAH,EAC. The positive expression rates of YKL-40 protein in NE,EAH,EAC tissues were 25% and 40%, respectively. The positive expression rate of YKL-40 protein in 76.8%.EAC was significantly higher than that in EAH (X2 + 9.034, P0. 0030. 05), and was also significantly higher than that in NE (X2 + 16. 984, P0. 0000. 05). The positive expression rate of YKL-40 protein in EAH was not significantly different from that in NE (X _ 2 ~ (2 +) ~ (1.026) P ~ (0.3110.05). The expression of YKL-40 protein in EAC was correlated with the histological grade of the tumor (P0. 008P 0.05), but not with age, clinical stage, myometrial invasion and lymph node metastasis (P0. 186 P0. 852P0. 511 P0. 752, P = 0. 752). P 0.05). 3. Spearman correlation analysis showed that there was a positive correlation (rs=0.367,P=0.005) between CD68 and YKL-40 protein expression in EAC tissues. Conclusion: 1. The positive expression rate of CD68 and YKL-40 protein in three groups of NE,EAH,EAC increased gradually. 2. With the decrease of tissue differentiation, myometrial infiltration and lymph node metastasis, the positive expression rate of CD68 protein was higher in EAC patients, and the positive rate of YKL-40 protein was higher with the decrease of tissue differentiation degree in EAC patients. 3. The overexpression of CD68 and YKL-40 protein in EAC tissues was positively correlated.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33
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