金雀异黄素对围绝经期小鼠卵巢和子宫组织中芳香化酶和FSHR的调控作用

发布时间：2018-11-11 11:21

【摘要】：目的:研究金雀异黄素对围绝经期模型小鼠卵巢和子宫组织中芳香化酶P450及促卵泡成熟激素受体(FSHR)的调节作用。方法 :选择12月龄自然衰老ICR小鼠作为围绝经期动物模型,分为对照组(CG),Gen低(L-Gen)、中(M-Gen)、高剂量组(H-Gen)及雌激素组(EG)。剂量组分别灌胃金雀异黄素(Gen)15,30,60 mg/kg,雌激素组灌胃戊酸雌二醇0.5 mg/kg,持续8周。RT-PCR及荧光定量PCR法测定小鼠卵巢和子宫中P450arom mRNA,CYP19 mRNA和FSHR的表达水平。结果 :与对照组相比,H-Gen和EG小鼠P450arom mRNA,CYP19mRNA和FSHR的表达量均升高,差异显著(P0.05)。结论 :金雀异黄素(60 mg/kg)可使小鼠卵巢与子宫中P450arom mRNA,CYP19 mRNA和FSHR的表达量升高,雌激素作用显著,揭示金雀异黄素可通过调节芳香化酶和促卵泡成熟激素受体基因来改善围绝经期卵巢与子宫的功能。
[Abstract]:Aim: to study the effects of genistein on aromatase P450 and follicle maturation hormone receptor (FSHR) in ovarian and uterine tissues of perimenopausal model mice. Methods: 12-month-old natural aging ICR mice were selected as perimenopausal animal models and divided into control group (L-Gen), moderate (M-Gen), high dose group (H-Gen) and estrogen group (EG). The estradiol valerate was perfused for 8 weeks with estradiol valerate 0.5 mg/kg, in the estrogen group. The expression of P450arom mRNA,CYP19 mRNA and FSHR in ovary and uterus of mice were measured by RT-PCR and fluorescence quantitative PCR method. Results: compared with the control group, the expression of P450arom mRNA,CYP19mRNA and FSHR in H-Gen and EG mice increased significantly (P0.05). Conclusion: genistein (60 mg/kg) can increase the expression of P450arom mRNA,CYP19 mRNA and FSHR in mouse ovary and uterus. It is suggested that genistein can improve the function of ovary and uterus by regulating aromatase and follicle stimulating hormone receptor gene.
【作者单位】：黑龙江八一农垦大学;哈尔滨医科大学;
【基金】：农业部农产加工品质量监督检验测试中心(大庆)工作站博士后资助课题(LRB13-153) 国家自然科学基金面上项目(81673170) 黑龙江省高校农产品加工与质量安全创新团队课题(2014TD006)
【分类号】：R1

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