妊娠糖尿病肾损伤早期尿NGAL和血清CysC的变化研究

发布时间：2018-11-23 08:28

【摘要】：目的探讨尿中性粒细胞明胶酶相关脂质运载蛋白(NGAL)和血清半胱氨酸蛋白酶抑制剂C(Cys C)对妊娠期糖尿病(GDM)肾损伤的早期变化及意义。方法收集新乡医学院第一附属医院85例GDM肾损伤血、尿液标本各5 m L,用酶联免疫吸附试验检测尿NGAL水平;同时用肌氨酸氧化酶法测定血清肌酐(Scr)水平;用免疫透射比浊法测定血清Cys C。结果 85例GDM组尿NGAL和血清Cys C测定结果明显升高,与正常孕妇组尿NGAL和血清Cys C比较,差异均有统计学意义(P0.05);GDM组与正常孕妇组血清Scr比较,Scr差异均无统计学意义(P0.05)。85例GDM血清Cys C高于正常者39例(45.88%),尿NGAL高于正常者53例(62.35%),两者联合检测任何一项高于正常者67例(78.82%),所以两者联合检测可以调高阳性率。结论尿NGAL和血清Cys C可作GDM早期肾损伤的诊断标志物。二者联合检测,可以提高阳性率。
[Abstract]:Objective to investigate the early changes and significance of urinary neutrophil gelatinase-associated lipid carrier protein (NGAL) and serum cysteine protease inhibitor (C (Cys C) on renal injury of gestational diabetes mellitus (GDM) with (GDM). Methods Eighty-five patients with renal injury of GDM were collected from the first affiliated Hospital of Xinxiang Medical College. Urine samples of 5 mL each were collected to detect the level of urinary NGAL by enzyme-linked immunosorbent assay (Elisa), and the serum creatinine (Scr) level was measured by creatine oxidase method. Serum Cys C was determined by immunoturbidimetric method. Results the levels of urinary NGAL and serum Cys C in 85 patients with GDM were significantly higher than those in normal pregnant women (P0.05). There was no significant difference in serum Scr between GDM group and normal pregnant women (P0.05). Serum Cys C in 85 cases of GDM was higher than normal in 39 cases (45.88%), urine NGAL was higher in 53 cases (62.35%). There were 67 cases (78.82%) with any higher than normal, so the positive rate could be adjusted by combined test. Conclusion urinary NGAL and serum Cys C can be used as diagnostic markers of early renal injury in GDM. The positive rate can be increased by combined detection of the two methods.
【作者单位】：新乡医学院第一附属医院检验科;新乡医学院第一附属医院输血科;新乡医学院医学检验学院;
【分类号】：R714.256

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