宫颈鳞癌EGFR、c-jun、c-fos表达与临床特征及近期疗效的关联性研究

发布时间：2018-12-27 08:42

【摘要】：目的：探讨宫颈鳞癌(CSCC)组织中表皮生长因子受体(EGFR)、原癌基因c-jun、c-fos表达与临床特征及近期疗效的关系,评估其在CSCC进展和近期疗效中的价值。方法：对60例CSCC组织标本采用荧光原位杂交(FISH)技术检测EGFR基因扩增及基因拷贝数；免疫组织化学法检测EGFR、c-jun、c-fos蛋白表达。采用卡方或确切概率、非参数Spearman等级相关、多因素Logistic回归等统计分析方法。结果：4种EGFR基因杂交模式所占比例分别为二倍体32.2%、三倍体25.4%、多倍体30.5%、基因扩增率11.9%；EGFR、c-jun、c-fos蛋白阳性表达率分别为70.0%、76.7%、68.3%；EGFR、c-jun、c-fos蛋白表达均与肿瘤分化程度、淋巴结转移有关(P0.05),EGFR蛋白表达还与临床分期有关(P=0.006),Spearman等级相关分析显示：EGFR蛋白表达水平与基因拷贝数增加呈显著正相关(r=0.622,P=0.000),c-jun、c-fos蛋白表达与临床分期呈正相关(r=0.293,P=0.023；r=0.296,P=0.022),EGFR与c-jun、c-fos蛋白表达水平呈正相关(r=0.422,P=0.001:r=0.509,P=0.000),EGFR、c-jun、c-fos蛋白表达对客观有效率RR无明显影响(P0.05)。结论：EGFR、c-jun、c-fos表达可能为CSCC高危进展、转移和预后的参考指标；EGFR、c-jun、c-fos表达均不是影响CSCC近期疗效的独立因素；c-jun、c-fos在EGFR介导的信号传导通路中可能发挥正性调节作用；联合检测EGFR、c-jun、c-fos为CSCC的诊断、个体化疗法提供理论依据。
[Abstract]:Objective: to investigate the relationship between the expression of proto-epidermal growth factor receptor (EGFR),) oncogene c-jun c-fos and its clinical features and short-term efficacy in cervical squamous cell carcinoma (CSCC), and to evaluate the value of c-junc-fos expression in the progression and short-term curative effect of CSCC. Methods: the amplification and copy number of EGFR gene were detected by fluorescence in situ hybridization (FISH) in 60 cases of CSCC and the expression of EGFR,c-jun,c-fos protein was detected by immunohistochemistry. Statistical analysis methods such as chi-square or exact probability, nonparametric Spearman rank correlation and multivariate Logistic regression were used. Results: the proportion of the four EGFR gene hybridization patterns was 32. 2 diploid, 25. 4 triploid, 35. 5 polyploid and 11. 9% gene amplification. The positive expression rates of EGFR,c-jun,c-fos protein were 70.0% and 76.78.3%, respectively. The expression of EGFR,c-jun,c-fos protein was related to the degree of tumor differentiation and lymph node metastasis (P0.05). The expression of), EGFR protein was also related to clinical stage (P0. 006). Spearman grade correlation analysis showed that there was a significant positive correlation between the expression level of EGFR protein and the increase of gene copy number (r = 0.622), while the expression of c-junn c-fos protein was positively correlated with the clinical stage (r = 0.293). There was a positive correlation between the expression of c-junn c-fos protein and the expression level of c-junn c-fos protein (0.296), EGFR). The expression of EGFR,c-jun,c-fos protein had no significant effect on the objective effective RR (P0.05). Conclusion: the expression of EGFR,c-jun,c-fos may be a reference index for the high risk progression, metastasis and prognosis of CSCC, and the expression of EGFR,c-jun,c-fos is not an independent factor affecting the short-term curative effect of CSCC. C-junn c-fos may play a positive role in the signal transduction pathway mediated by EGFR, and the combined detection of EGFR,c-jun,c-fos may provide theoretical basis for the diagnosis of CSCC and individualized therapy.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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