NF-κBp65与妊娠期糖尿病的相关性研究
发布时间：2019-01-23 13:19
【摘要】：目的通过对妊娠期糖尿病孕妇血清、脐血中NF-κBp65的检测及胎盘组织中核因子NF-KBp65的表达,评估和分析妊娠期糖尿病与核因子NF-KBp65两者的相关性。为妊娠期糖尿病的发病机制提供理论依据,并为糖尿病的预防及治疗提供参考。 材料与方法收集2011年11月至2013年3月在天津市中心妇产科医院住院孕产妇(妊娠期糖尿病的孕妇及正常妊娠的孕妇)作为研究组,正常未孕女性作为对照组,依据IADPSG2010年推荐的GDM诊断标准将研究分为3组,分别为诊断为妊娠期糖尿病的孕妇组(Ⅰ组,n=38)、正常孕妇组(Ⅱ组,n=56)、正常未孕组(Ⅲ组,n=63)。抽取受试者产前清晨空腹肘静脉血3ml,正常未孕女性于门诊取血,Ⅰ组和Ⅱ组孕妇分娩时于胎盘娩出后1min内在脐带根部抽取脐静脉血3ml,采用酶联免疫吸附检测Ⅰ组和Ⅱ组研究对象血清、脐血中NF-κBp65的表达,Ⅲ组研究对象血清中NF-KBp65的表达。Ⅰ组和Ⅱ组孕妇分娩时在胎盘娩出后10mmin内,无菌状态下,于胎盘母体面脐带附着处(避开出血、梗死及钙化区)垂直剪取2cm×2cm大小胎盘全层组织,采用免疫组化法测定研究对象中胎盘组织中NF-KBp65的表达。资料进行统计学分析,计量资料采用均数±标准差(x±s)表示,均数比较时,多组均数比较采用方差分析,两组间比较采用独立样本t检验或秩和检验。计数资料采用χ2检验。变量间进行Pearson相关分析。P0.05为差异具有统计学意义。 结果 1.妊娠期糖尿病孕妇组母血和脐血中NF-KBp65水平均高于正常孕妇组,差异具有统计学意义(P=0.000,0.000,P0.01)。 2.正常孕妇组与正常未孕女性组血清中NF-KBp65水平比较,差异无统计学意义(P=0.270,P0.05)。 3.妊娠期糖尿病孕妇组(r=0.710,P=0.026,P0.05),母血NF-κBp65水平与脐血NF-KBp65水平有关联,呈直线正相关。 4. NF-κBp65在妊娠期糖尿病孕妇组和正常孕妇组胎盘组织中的表达阳性率分别为100.00%,12.5%,差异具有统计学意义(χ2=69.456,P=0.000,P0.01)。 结论(1)妊娠期糖尿病孕妇其血清及脐血中NF-κBp65水平较正常孕妇显著升高,正常孕妇血清NF-κBp65水平较正常育龄未孕女性无明显升高,提示NF-κBp65可能参与了妊娠期糖尿病的发生发展,而怀孕因素对血清NF-κBp65水平无显著影响。(2)妊娠期糖尿病孕妇组母血NF-κBp65水平与脐血NF-κBp65水平间呈直线正相关,提示母血中NF-κBp65水平升高可能是诱导脐血中NF-κBp65水平升高的重要原因之一,推测可能造成巨大儿、大于胎龄儿等的发生。(3)妊娠期糖尿病孕妇胎盘组织中NF-κBp65的表达阳性率较正常孕妇组显著升高,提示NF-κBp65有可能在妊娠期糖尿病的病理生理过程中发挥了一定作用。
[Abstract]:Objective to evaluate and analyze the correlation between gestational diabetes mellitus (GDM) and nuclear factor NF-KBp65 (NF-KBp65) by detecting NF- 魏 Bp65 in serum and umbilical cord blood and the expression of nuclear factor NF-KBp65 in placenta tissue of pregnant women with gestational diabetes mellitus (GDM). To provide theoretical basis for the pathogenesis of gestational diabetes mellitus, and to provide a reference for the prevention and treatment of diabetes mellitus. Materials and methods from November 2011 to March 2013, hospitalized pregnant women (pregnant women with gestational diabetes mellitus and pregnant women with normal pregnancy) in Tianjin Obstetrics and Gynecology Hospital of the city center of Tianjin were selected as study group and normal non-pregnant women as control group. According to the diagnostic criteria of GDM recommended by IADPSG2010, the study was divided into three groups: pregnant women diagnosed as gestational diabetes mellitus (group 鈪，
本文编号：2413812
[Abstract]:Objective to evaluate and analyze the correlation between gestational diabetes mellitus (GDM) and nuclear factor NF-KBp65 (NF-KBp65) by detecting NF- 魏 Bp65 in serum and umbilical cord blood and the expression of nuclear factor NF-KBp65 in placenta tissue of pregnant women with gestational diabetes mellitus (GDM). To provide theoretical basis for the pathogenesis of gestational diabetes mellitus, and to provide a reference for the prevention and treatment of diabetes mellitus. Materials and methods from November 2011 to March 2013, hospitalized pregnant women (pregnant women with gestational diabetes mellitus and pregnant women with normal pregnancy) in Tianjin Obstetrics and Gynecology Hospital of the city center of Tianjin were selected as study group and normal non-pregnant women as control group. According to the diagnostic criteria of GDM recommended by IADPSG2010, the study was divided into three groups: pregnant women diagnosed as gestational diabetes mellitus (group 鈪，
本文编号：2413812
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