上皮性卵巢癌组织中miR-126、EGFL7的表达与微血管密度的检测

发布时间：2019-01-23 14:07

【摘要】：目的:研究miR-126、类表皮生长因子域7(EGFL7)在上皮性卵巢癌组织中的表达情况及与微血管密度(MVD)的关系。方法:采用qRT-PCR法检测21例正常卵巢组织(正常组)、23例良性卵巢肿瘤组织(良性组)及45例上皮性卵巢癌组织(恶性组)中miR-126、EGFL7 mRNA表达水平,采用免疫组化法检测3种组织中EGFL7蛋白表达情况及MVD。结果:miR-126 mRNA在恶性组中表达水平低于正常组、良性组(H=43.611,P0.001);恶性组EGFL7蛋白阳性表达率、MVD高于良性组及正常组(χ2=33.629,F=262.292,P均0.001)。miR-126、EGFL7mRNA表达及MVD均与上皮性卵巢癌FIGO分期、淋巴结转移有关(P均0.05)。上皮性卵巢癌组织中miR-126与EGFL7、MVD呈负相关(r=-0.413、-0.909,P0.05),EGFL7与MVD呈正相关(r=0.362,P=0.015)。结论:miR-126、EGFL7可能与上皮性卵巢癌的发生发展、侵袭转移过程有关。
[Abstract]:Aim: to investigate the expression of epidermal growth factor domain 7 (EGFL7) in epithelial ovarian carcinoma and its relationship with microvessel density (MVD). Methods: the expression of miR-126,EGFL7 mRNA in 21 normal ovarian tissues (normal group), 23 benign ovarian tumor tissues (benign group) and 45 epithelial ovarian carcinoma tissues (malignant group) was detected by qRT-PCR method. Expression of EGFL7 protein and MVD. in three kinds of tissues were detected by immunohistochemical method Results: the expression of miR-126 mRNA in malignant group was lower than that in normal group and benign group (P 0.001). The positive expression rate of EGFL7 protein in malignant group was higher than that in benign group and normal group (蠂 ~ 2 ~ 2 ~ (33.629) F ~ (262.292) P ~ (0.001). The expression of miR-126,EGFL7mRNA and MVD were related to FIGO stage and lymph node metastasis of epithelial ovarian carcinoma (P < 0. 05). There was a negative correlation between miR-126 and EGFL7,MVD in epithelial ovarian carcinoma (r = 0.413) and a positive correlation between EGFL7 and MVD (r = 0.362). Conclusion: miR-126,EGFL7 may be related to the development, invasion and metastasis of epithelial ovarian cancer.
【作者单位】：郑州大学第三附属医院妇瘤科;郑州大学第三附属医院病理科;
【分类号】：R737.31

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