VEGF、MMP-2和TNF-α在子宫肌瘤组织中的表达及临床意义

发布时间：2019-05-30 04:25

【摘要】：目的检测血管内皮生长因子(VEGF)、基质金属蛋白酶(MMP)-2和肿瘤坏死因子(TNF)-α在人子宫肌瘤中的表达,探讨其在子宫肌瘤发生、发展的作用。方法取2013年1~12月该院收治的54例子宫肌瘤患者的子宫肌瘤组织和正常子宫肌组织,分别采用免疫组化法测定雌激素受体(ER)和孕激素受体(PR)的表达,采用RT-PCR法测定VEGF、MMP-2和TNF-αmRNA的表达,比较子宫肌瘤组织和正常子宫肌组织表达的差异,并且分析ER、PR与VEGF、MMP-2、TNF-α表达的相关性。结果 (1)子宫肌瘤组织ER和PR的阳性表达率分别为68.52%和75.93%,均显著高于正常子宫肌组织(P0.05或P0.01);(2)子宫肌瘤组织中VEGF、MMP-2和TNF-αmRNA表达水平均较正常子宫肌组织显著升高(P0.01);(3)ER(+)和PR(+)子宫肌瘤组织VEGF、MMP-2和TNF-αmRNA表达较ER(-)和PR(-)子宫肌瘤组织均显著升高(P0.01);(4)相关性分析显示,ER、PR表达评分均与VEGF、MMP-2和TNF-αmRNA表达水平呈正相关(P0.01)。结论 VEGF、MMP-2和TNF-α在子宫肌瘤均高表达,在其发生、发展中起重要作用,并且ER和PR的高表达相关。
[Abstract]:Objective to detect the expression of vascular endothelial growth factor (VEGF), matrix metalloprotease (MMP)-2 and tumor necrosis factor (TNF)-伪 in human uterine myoma and to explore the role of vascular endothelial growth factor (MMP)-2 and tumor necrosis factor (TNF)-伪 in the occurrence and development of uterine myoma. Methods from January to December 2013, the expressions of estrogen receptor (ER) and progesterone receptor (PR) were measured in 54 patients with uterine myoma and normal uterine muscle tissues. The expression of VEGF,MMP-2 and TNF- 伪 mRNA was detected by RT-PCR method, and the difference of expression between uterine myoma tissue and normal uterine muscle tissue was compared, and the correlation between ER,PR and VEGF,MMP-2,TNF- 伪 expression was analyzed. Results (1) the positive expression rates of ER and PR in uterine myoma were 68.52% and 75.93%, respectively, which were significantly higher than those in normal uterine muscle (P 0.05 or P 0.01). (2) the expression of VEGF,MMP-2 and TNF- 伪 mRNA in uterine myoma was significantly higher than that in normal uterine muscle (P 0.01). (3) the expression of VEGF,MMP-2 and TNF- 伪 mRNA in) ER () and PR () hysteromyoma tissues was significantly higher than that in ER (-) and PR (-) hysteromyoma tissues (P 0.01). (4) correlation analysis showed that ER,PR expression score was positively correlated with the expression of VEGF,MMP-2 and TNF- 伪 mRNA (P 0.01). Conclusion both VEGF,MMP-2 and TNF- 伪 are highly expressed in uterine myoma and play an important role in its occurrence and development, and the high expression of ER and PR is related.
【作者单位】：唐山市开滦(集团)有限责任公司唐家庄医院;开滦总医院;
【基金】：河北省科学技术厅课题(20132924)
【分类号】：R737.33

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