评估无血清培养子宫内膜间充质干细胞的可行性

发布时间：2018-06-15 17:55

本文选题：子宫内膜间充质干细胞 + 细胞增殖　；参考：《解放军医学院学报》2015年06期

【摘要】：目的使用无血清培养基体外分离扩增子宫内膜间充质干细胞,研究其生物学特性。方法比较在无血清培养基中和含10%胎牛血清培养基中子宫内膜间充质干细胞细胞形态细胞表型、增殖能力、细胞活率和分化能力等生物学特性。结果无血清培养基和有血清培养基培养的子宫内膜间充质干细胞形态相似,但是前者呈明显的漩涡状生长,更加细长,立体感更强;无血清和有血清培养的子宫内膜间充质干细胞表面标记物均呈阳性;无血清培养的子宫内膜间充质干细胞细胞活率更高、细胞增殖能力更强。结论无血清培养基能够在体外扩增子宫内膜间充质干细胞,并使其生物学特性(细胞增殖,细胞活率)优于胎牛血清培养的子宫内膜间充质干细胞,且分化能力无改变,可以取代胎牛血清用于细胞治疗,避免有血清培养的干细胞治疗引起的人畜共患病及免疫原性反应。
[Abstract]:Objective to isolate and amplify endometrial mesenchymal stem cells in serum-free medium in vitro and study the biological characteristics of endometrial mesenchymal stem cells. Methods the morphology, proliferation, viability and differentiation of endometrial mesenchymal stem cells in serum-free medium and 10% fetal bovine serum medium were compared. Results the morphology of endometrial mesenchymal stem cells cultured in serum-free medium was similar to that in serum-free medium, but the former had obvious whirlpool growth, more slender and more stereosensitive. The surface markers of both serum-free and serum-free endometrial mesenchymal stem cells were positive, and the serum-free endometrial mesenchymal stem cells had higher viability and stronger proliferative ability. Conclusion Serum-free medium can amplify endometrial mesenchymal stem cells in vitro, and its biological characteristics (cell proliferation, cell viability) are better than that of fetal bovine serum cultured endometrial mesenchymal stem cells, and the differentiation ability of endometrial mesenchymal stem cells does not change. It can replace fetal bovine serum for cell therapy and avoid zoonosis and immunogenicity caused by stem cell therapy.
【作者单位】：解放军医学院;海军总医院妇产科;
【基金】：国家“十二五”科技支撑计划项目(2012BA1321305) 国家自然科学基金项目(81000245;81370703) 全军后勤科研课题(CHJ13,1012)~~
【分类号】：R329

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