人脐带间充质干细胞生物学特性及脉冲电磁场促进其成骨分化研究

发布时间：2018-07-12 13:11

  本文选题：人脐带间充质干细胞 + 成骨细胞　； 参考：《吉林大学》2010年博士论文

【摘要】： 间充质干细胞是一群中胚层来源的具有自我更新和多向分化潜能的多能干细胞。来自于胎儿期的间充质干细胞具有更强大的增殖能力、更广谱的分化潜能、更低的免疫原性等。有望作为组织工程或细胞替代治疗优良的种子细胞。 本实验从流产胎儿(胎龄12～18周)脐带沃顿胶中分离人脐带间充质干细胞,对其形态学、细胞周期、生长动力学、免疫表型及多分化潜能(向脂肪细胞、软骨细胞、成骨细胞等分化)等生物学特性进行研究,证实所分离的细胞为间充质干细胞。 以脉冲电磁场作为刺激hUCMSCs成骨分化的手段,比较脉冲电磁场方式和化学诱导方式对hUCMSCs分化为成骨细胞的速度、基因表达等方面的差异,明确PEMFs刺激hUCMSCs体外成骨分化能力;建立兔挠骨缺损模型,hUCMSCs与羟基磷灰石混合后注入兔挠骨缺损处,PEMFs每天刺激3h,观察骨缺损再生情况,探讨PEMFs促进hUCMSCs体内成骨分化能力。从而为骨组织工程和细胞替代治疗提供新的种子细胞来源;证实PEMFs促进hUCMSCs成骨分化的可行性。本实验研究结果为实现hUCMSCs的常规化分离培养提供了有价值的实验依据;为验证hUCMSCs具有多分化潜能提供有力依据;为证实PEMFs是促进hUCMSCs成骨分化的有效手段提供了实验依据;为PEMFS诱导hUCMSCs向成骨细胞分化的临床应用提供理论依据和技术支持。
[Abstract]:Mesenchymal stem cells are a group of mesoderm derived pluripotent stem cells with self-renewal and multi-differentiation potential. Mesenchymal stem cells from fetal stage have stronger proliferative ability, wider differentiation potential, lower immunogenicity and so on. It is expected to be a good seed cell for tissue engineering or cell replacement therapy. In this study, human umbilical cord mesenchymal stem cells were isolated from aborted fetus (12 ~ 18 weeks of gestational age) with Wharton gum. Morphology, cell cycle, growth kinetics, immunophenotype and multi-differentiation potential (adipocytes, chondrocytes) of human umbilical cord mesenchymal stem cells were studied. The biological characteristics of osteoblasts were studied, and the isolated cells were confirmed as mesenchymal stem cells. Using pulsed electromagnetic field as the means to stimulate the osteogenic differentiation of hUCMSCs, the difference of the speed and gene expression of pulsed electromagnetic field and chemical induction on the differentiation of hUCMSCs into osteoblasts in vitro was compared, and the ability of PEMFs to stimulate the osteogenic differentiation of hUCMSCs in vitro was determined. The rabbit model of scratching bone defect was established, and then injected with PEMFs into rabbit flexor bone defect for 3 hours every day. The regeneration of bone defect was observed, and the ability of PEMFs to promote osteogenic differentiation of hUCMSCs in vivo was investigated. These results provide a new source of seed cells for bone tissue engineering and cell replacement therapy, and confirm the feasibility of PEMFs in promoting osteogenic differentiation of hUCMSCs. The results of this study provide valuable experimental basis for the conventional isolation and culture of hUCMSCs, provide a powerful basis for verifying the multi-differentiation potential of hUCMSCs, and provide experimental basis for confirming that PEMFs are an effective means to promote the osteogenic differentiation of hUCMSCs. To provide theoretical basis and technical support for the clinical application of PEMFS induced differentiation of hUCMSCs into osteoblasts.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2010
【分类号】：R329

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