神经轴突导向因子1裸质粒转染对5/6肾切除模型大鼠肾脏管周毛细血管网的影响（英文）
发布时间：2021-11-11 17:53
背景:神经轴突导向因子1作为血管内皮细胞的促有丝分裂原,能够促进血管内皮细胞的迁移和增殖,发挥诱导血管新生的作用。目的:观察神经轴突导向因子1裸质粒转染对5/6肾切除大鼠模型残余肾功能的保护及对肾小管周围毛细血管网的影响。方法:将30只SD大鼠随机等分为假手术组、模型组和治疗组。模型组和治疗组大鼠切除左侧肾脏上、下极各1/3,1周后切除右肾,建立残肾模型。模型组和治疗组大鼠于切除右肾的同时在左肾静脉分别注射空质粒IRES2-EGFP和pC MV6-XL5-Netrin-1-IRES2-EGFP。结果与结论:与模型组相比,治疗组大鼠血尿素氮、血肌酐水平降低,肾间质纤维化程度减轻,肾小管周围毛细血管网的密度增加,肾小管胞浆中神经轴突导向因子1蛋白表达增加。提示神经轴突导向因子1裸质粒转染能明显改善5/6肾切除模型大鼠的肾功能,减轻残肾组织的病理损害和肾间质纤维化面积,增加肾小管周围毛细血管网密度、降低缺氧诱导因子1α表达,从而改善肾间质小管缺氧状态。
【文章来源】：中国组织工程研究. 2015,19(24)北大核心
【文章页数】：8 页
【文章目录】：
INTRODUCTION
MATERIALS AND METHODS
Design
Time and setting
Materials
Methods
Preparation of p CMV-NETRIN-1-IRES2-EGFP plasmids
Establishment of 5/6 nephrectomized rat models and grouping
Gene transfection
Sample harvesting
Morphological examination of the renal tissues
Immunohistochemistry staining
Western blot determination
Main outcome measurements
Statistical analysis
RESULTS
Quantitative analysis of experimental animals
Transfection effectiveness of p CMV-NETRIN-1-IRES2-EGFP
Renal function and 24-hour urine protein
Pathological changes of the rat kidney
Peritubular capillary network density
Expression of netrin-1 and hypoxia inducible factor 1αin the renal tissues of rats
DISCUSSION
【参考文献】：
期刊论文
[1]NRF-1基因真核慢病毒表达载体的构建及表达[J]. 宋熔,王程铖,王强,王玉姣,李君良,赵巍. 心脏杂志. 2014(03)
[2]Diabetic nephropathy:Treatment with phosphodiesterase type 5 inhibitors[J]. Cecil Stanley Thompson. World Journal of Diabetes. 2013(04)
[3]Netrin-1在胎儿生长受限及重度子痫前期胎盘中的表达[J]. 钟少平,刘霞,周晓宇,金小英,彭华,邹丽. 中国妇幼保健. 2012(34)
[4]氯沙坦对肾间质纤维化的抑制作用[J]. 孙蔚楠,卢钊宇,谢院生,崔少远,丁瑞,邱强,傅博,耿文佳,刘旭生,陈香美. 中国中西医结合肾病杂志. 2012(05)
[5]血、尿胱抑素C在糖尿病肾病早期检测中的应用价值[J]. 陈正徐,王莉,李曼. 安徽医药. 2011(10)
[6]单侧输尿管梗阻模型小鼠肾脏微血管损伤与肾间质纤维化的关系研究[J]. 韩红,孙东,麻艳艳,尹忠诚. 中国病理生理杂志. 2010(12)
[7]Effect of Different Concentrations of Neogenin on Proliferation,Apoptosis and Related Proliferative Factors in Human Trophoblasts[J]. 钟少平,邹丽,赵茵,胡彬,谢涵. Journal of Huazhong University of Science and Technology（Medical Sciences）. 2010(04)
[8]维拉帕米和超氧化物歧化酶对体外冲击波肾损伤的保护作用[J]. 林宗明,方银忠,张永康,邹秋锋,唐群业,王晨曦. 中华泌尿外科杂志. 2003(02)
本文编号：3489264
【文章来源】：中国组织工程研究. 2015,19(24)北大核心
【文章页数】：8 页
【文章目录】：
INTRODUCTION
MATERIALS AND METHODS
Design
Time and setting
Materials
Methods
Preparation of p CMV-NETRIN-1-IRES2-EGFP plasmids
Establishment of 5/6 nephrectomized rat models and grouping
Gene transfection
Sample harvesting
Morphological examination of the renal tissues
Immunohistochemistry staining
Western blot determination
Main outcome measurements
Statistical analysis
RESULTS
Quantitative analysis of experimental animals
Transfection effectiveness of p CMV-NETRIN-1-IRES2-EGFP
Renal function and 24-hour urine protein
Pathological changes of the rat kidney
Peritubular capillary network density
Expression of netrin-1 and hypoxia inducible factor 1αin the renal tissues of rats
DISCUSSION
【参考文献】：
期刊论文
[1]NRF-1基因真核慢病毒表达载体的构建及表达[J]. 宋熔,王程铖,王强,王玉姣,李君良,赵巍. 心脏杂志. 2014(03)
[2]Diabetic nephropathy:Treatment with phosphodiesterase type 5 inhibitors[J]. Cecil Stanley Thompson. World Journal of Diabetes. 2013(04)
[3]Netrin-1在胎儿生长受限及重度子痫前期胎盘中的表达[J]. 钟少平,刘霞,周晓宇,金小英,彭华,邹丽. 中国妇幼保健. 2012(34)
[4]氯沙坦对肾间质纤维化的抑制作用[J]. 孙蔚楠,卢钊宇,谢院生,崔少远,丁瑞,邱强,傅博,耿文佳,刘旭生,陈香美. 中国中西医结合肾病杂志. 2012(05)
[5]血、尿胱抑素C在糖尿病肾病早期检测中的应用价值[J]. 陈正徐,王莉,李曼. 安徽医药. 2011(10)
[6]单侧输尿管梗阻模型小鼠肾脏微血管损伤与肾间质纤维化的关系研究[J]. 韩红,孙东,麻艳艳,尹忠诚. 中国病理生理杂志. 2010(12)
[7]Effect of Different Concentrations of Neogenin on Proliferation,Apoptosis and Related Proliferative Factors in Human Trophoblasts[J]. 钟少平,邹丽,赵茵,胡彬,谢涵. Journal of Huazhong University of Science and Technology（Medical Sciences）. 2010(04)
[8]维拉帕米和超氧化物歧化酶对体外冲击波肾损伤的保护作用[J]. 林宗明,方银忠,张永康,邹秋锋,唐群业,王晨曦. 中华泌尿外科杂志. 2003(02)
本文编号：3489264
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