睾酮通过细胞膜雄激素受体影响骨髓巨噬细胞中Notch受体及配体的表达

发布时间：2023-02-11 14:46

　　1.1.1.研究背景 雄激素(androgen)主要指睾酮(testosterone, T)和其在体内的活性代谢产物双氢睾酮(dihydrotestosterone, DHT)。睾酮由睾丸Leydig细胞产生并分泌,其合成和分泌过程受下丘脑-垂体-性腺轴及其它神经体液因素的严密调控。新生成的睾酮进入血液循环后,大部分与性激素结合球蛋白(sex hormone binding globulin, SHBG)结合形成复合物,不能进入组织和细胞;只有约1-2%呈游离状态(血浆浓度约为19.9±4.7nM),能为组织所利用,发挥内分泌调节作用。组织和细胞中的5α-还原酶可将睾酮还原成双氢睾酮,后者与雄激素受体的亲和力要高于睾酮本身。雄激素在生殖系统的作用是:促进胚

【文章页数】：85 页

【学位级别】：博士

【文章目录】：
Part 1 Abstracts
    1.1 Abstracts in Chinese
        1.1.1. Research background
        1.1.2. Resarch purpose
        1.1.3. Research protocals
        1.1.4. Results
        1.1.5. Conclusion
        1.1.6. Key wprds
    1.2. Abstracts in English
Part 2 Introduction
Part 3 Methods and Materials
    3.1. Mice
    3.2. Cell cultures
    3.3. Purity assay of BMMs
    3.4. Treatment of BMMs
    3.5. Reverse-transcriptase polymerase chain reaction (RT-PCR)
    3.6. Western blotting assay
    3.7. Determination of [Ca²⁺]
    3.8. Visualization of intracellular androgen receptor
    3.9. Labeling with testosterone-BSA-FITC
    3.10. Confocal laser scanning microscopy assay
    3.11. Statistical analysis
Part 4 Results
    4.1. Assessment of the Purity of BMMs
    4.2. Expression analysis of Notch in testosterone-treated BMMs
    4.3. Regulations of testosterone on LPS-induced Notch expression
    4.4. Testosterone-induced Ca²⁺ mobilization
    4.5. Absence of androgen receptor
    4.6. Testosterone binding sites on BMMs
Part 5 Discussion
    5.1. Effects of testosterone on BMMs
    5.2. Mechanism of testosterone action in macrophages
Part 6 Conclusion
Part 7 References
Part 8 Achievements (in Chinese)
Part 9 Review
    9.1. Secretion and production of androgen
    9.2. Biological effects of androgen
    9.3. Testosterone and immune response
    9.4. Testosterone and macrophage
    9.5. Mechanism of testosterone action
    9.6. Receptors for Rapid Androgen Signaling
    9.7. Members of Notch signaling pathway
    9.8 Perspectives
Part 10 Statement
Part 11 Acknowledgements



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