spvB/spvC基因对沙门菌毒力及宿主免疫功能的影响

发布时间：2024-05-17 13:10

　　目的研究spv B/spv C基因对沙门菌毒力及对宿主免疫系统的影响。方法野生型沙门菌(STM.211)、敲除spv B基因的沙门菌(STM.211-Δspv B)、敲除spv C基因的沙门菌(STM.211-Δspv C)、敲除spv B及spv C基因的沙门菌(STM.211-Δspv B.spv C)和PBS组分别经腹腔注射0.2 m L105CFU的STM.211、STM.211-Δspv B、STM.211-Δspv C、STM.211-Δspv B.spv C感染BALB/c小鼠,观察小鼠精神状态、运动情况、腹泻、体质量、毛发改变等感染中毒症状,用ELISA法测定小鼠血清IL-10、IL-12、IFN-γ水平;并分离小鼠肝、脾,观察靶器官大体观及显微镜下的病理改变。结果(1)STM.211、STM.211-Δspv B和STM.211-Δspv C组的中毒症状明显较PBS组严重,STM.211-Δspv B.spv C组与PBS组间无明显差异;(2)STM.211组、STM.211-Δspv B组、STM.211-Δspv C组的IFN-γ和IL-12分泌量均显著低于STM...

【文章页数】：6 页

【部分图文】：

图1小鼠肝脏大体观改变Fig.1Generalviewchangesoftheliver(3d).A:PBS;B:STM.211;C:STM.211-ΔspvB;D:STM.211-ΔspvC;E:STM.211-ΔspvB.spvC.Theliverofmicewhichinfectedwithsalmonellaareincreasedandcongestion,anddottednecroticABCDE

-ΔspvB、STM.211-spvCSTM.211-ΔspvB.spvC>PBS。2.2小鼠肝脏和脾脏的病理变化感染沙门菌后1d，各组间肝脾饱满有光泽，大小与正常对照组无明显差异。感染后3d起，分离小鼠肝脾，肉眼观察发现感染沙门菌的小鼠的肝脾明显增大并充血，肝脏表面出现点状坏死....

图2小鼠脾脏大体观改变Fig.2Generalviewchangesofthespleen(3d).A:PBS;B:STM.211;C:STM.211-ΔspvB;D:STM.211-ΔspvC;E:STM.211-ABCDE

图3小鼠肝脏光镜下病理改变Fig.3Lightmicroscopyviewchangesoftheliver,3d（HE,Originalmagnification:×40）.A:PBS;B:STM.211;C:STM.211-ΔspvB;D:STM.211-ΔspvC;E:STM.211-ΔspvB.spvC.Thehepatocyteishydropicdegeneration,anditscollectabbacyinflammatorycellsinfiltrationandfocalpointofflakenecrosis.Thedegreeofthepathologicalchanges:STM.211＞DE

图4小鼠脾脏光镜下病理改变，3dFig.4Lightmicroscopyviewchangesofthespleen,3d（HE,Originalmagnification:×20）.A:PBS;B:STM.211;C:STM.211-ΔspvB;D:STM.211-ΔspvC;E:STM.211-ΔspvB.spvC.Thespleenhavemultinucleatedgiantcellhyperplasia，andthereisnoclearboundarybetweencortexandmedulla，splenicsinusexpansionhyperemia.Thedegreeofthepathological

，与IL-12共同诱导TH0细胞向TH1细胞分化，增强细胞免疫。缺乏IFN-γ有利于细菌繁殖，使宿主图3小鼠肝脏光镜下病理改变Fig.3Lightmicroscopyviewchangesoftheliver,3d（HE,Originalmagnification:×40）.A:....

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