膝关节炎软骨基质金属蛋白酶2、基质金属蛋白酶9表达与MRI表现的相关性

发布时间：2018-03-01 08:03

本文关键词： 骨关节炎基质金属蛋白酶基质金属蛋白酶磁共振成像组织工程组织构建骨组织工程膝关节炎 MRI 相关性　出处：《中国组织工程研究》2016年37期 　论文类型：期刊论文

【摘要】：背景:研究表明,基质金属蛋白酶可降解关节骨与软骨中的胶原、明胶、弹性蛋白、蛋白多糖等细胞外基质大分子,破坏了细胞外基质产生与降解的平衡,是风湿性关节骨及软骨的破坏的重要原因。目的:检测了兔膝关节炎软骨基质金属蛋白酶2、基质金属蛋白酶9的表达,分析了两者与MRI表现的相关性。方法:选取健康无骨关节炎表现的日本大耳白兔50只,随机分为正常对照组10只和模型组40只。模型组采用前后交叉韧带切断术建立骨关节炎模型,分别在造模后第1,3,5与7周(各10只),进行MRI检查和软骨基质金属蛋白酶2、基质金属蛋白酶9表达水平测定。结果与结论:(1)MRI检查结果显示,骨关节炎模型兔的软骨破坏日趋明显,MRI分级差异显著(P0.05);(2)模型组软骨基质金属蛋白酶2与基质金属蛋白酶9 mR NA表达明显高于对照组(P0.05),基质金属蛋白酶2表达水平在造模后第3周最高(P0.01),随后2周略有下降,但仍显著高于对照组(P0.05);基质金属蛋白酶9在第5周达到最高表达水平(P0.01),第7周模型组基质金属蛋白酶9表达水平下降,但与对照组相比仍有显著性意义(P0.05);(3)各组软骨损伤的MRI分级与基质金属蛋白酶2(R2=0.119,P=0.012)、基质金属蛋白酶9表达皆存在相关性(R2=0.466,P=0.037)。(4)结果说明,血清基质金属蛋白酶2、基质金属蛋白酶9水平与骨关节炎患者的病情活动及早期关节软骨破坏显著相关,可以作为早期骨关节炎患者的预测指标。
[Abstract]:Background: studies have shown that matrix metalloproteinases can degrade extracellular matrix macromolecules such as collagen, gelatin, elastin and proteoglycan in articular bone and cartilage, thus disrupting the balance between the production and degradation of extracellular matrix. Objective: to detect the expression of matrix metalloproteinase 2 and matrix metalloproteinase 9 in rabbit knee arthritis. Methods: 50 Japanese large ear white rabbits with healthy osteoarthritis were selected. The models of osteoarthritis were established by anterior and posterior cruciate ligament resection in the model group (n = 10) and the model group (n = 40). MRI examination and expression of matrix metalloproteinase 2 and matrix metalloproteinase 9 were performed at 5 and 7 weeks after modeling. The cartilage damage of osteoarthritis model rabbits was more and more obvious. The difference of MRI grade was significant (P 0.05). The expression of matrix metalloproteinase 2 and matrix metalloproteinase 9 mR na in the model group was significantly higher than that in the control group. The expression level of matrix metalloproteinase 2 was significantly higher than that in the control group. In the third week, the peak value of P0.01G was decreased slightly in the second week after the model was made. The expression level of matrix metalloproteinase 9 reached the highest level at the 5th week and decreased in the model group at the 7th week, but it was still significantly higher than that in the control group, and the expression level of matrix metalloproteinase 9 was significantly higher than that of the control group. However, compared with the control group, there were still significant differences in the MRI grade and matrix metalloproteinase (MMP) 2R _ 2O _ (0.119) P _ (0.012), and the expression of matrix metalloproteinase 9 was correlated with the expression of matrix metalloproteinase _ 9 (P _ (0.466) P _ (0.037) ~ (4)). The serum levels of matrix metalloproteinase 2 and matrix metalloproteinase 9 were significantly correlated with the disease activity and the early destruction of articular cartilage in patients with osteoarthritis and could be used as predictors of early osteoarthritis.
【作者单位】：青海大学附属医院创伤骨科;
【分类号】：R684.3

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