大鼠脊髓损伤后形态学观察和LIMK1表达的研究

发布时间：2018-03-01 18:38

本文关键词： 脊髓损伤 LIM结构域激酶胶质纤维酸性蛋白　出处：《组织工程与重建外科杂志》2016年06期 　论文类型：期刊论文

【摘要】：目的通过监测大鼠脊髓损伤后其形态学和LIMK1、GFAP蛋白表达水平的动态改变,对脊髓损伤后LIMKL1是否参与胶质瘢痕的形成及调控进行初步研究。方法 Wistar成年大鼠54只,随机分为3组:对照组、假伤组和SCI组,SCI组采用改良Allen's致伤装置制作大鼠脊髓损伤模型,采用BBB运动功能评分法观察大鼠后肢功能恢复情况。分别于术后1、2、3、4周取材,通过HE染色、免疫组化、免疫荧光等方法,观察伤后不同时期脊髓的病理变化及LIMK1、GFAP的表达情况。结果 SCI组死亡率33%,BBB评分示SCI组术后2周后肢运动功能有明显恢复(P0.01)。LIMK1、GFAP的免疫组化和激光共聚焦显微镜分析结果基本一致,两者蛋白水平表达均于伤后1周增高。GFAP表达于3周达峰值,4周时开始下降;LIMK1在伤后1周时出现峰值,2周开始波动下降,3周时达峰值,4周开始下降。结论大鼠脊髓损伤后,LIMK1和GFAP表达密切相关,结合LIMK1蛋白表达谱,提示LIMK1通路参与胶质瘢痕的形成和调控。
[Abstract]:Objective to investigate whether LIMKL1 is involved in the formation and regulation of glial scar after spinal cord injury (sci) in rats by monitoring the dynamic changes of morphology and LIMK1 GFAP protein expression level. Methods 54 adult Wistar rats were involved in the formation and regulation of glial scar. Rats were randomly divided into three groups: control group, sham injury group and SCI group. The spinal cord injury model of rats was made with modified Allen's injury device. The functional recovery of hind limbs was observed by BBB motor function scoring method. By means of HE staining, immunohistochemistry, immunofluorescence and other methods, The pathological changes of spinal cord and the expression of LIMK1 + GFAP were observed at different time after injury. Results the mortality rate of SCI group was 33 BBB score. Results the results of immunohistochemistry and laser confocal microscopy analysis showed that the motor function of hind limbs in SCI group was significantly recovered 2 weeks after operation. The expression of GFAP increased at 1 week after injury. The expression of GFAP began to decrease at the end of 3 weeks and the peak value of LIMK1 at 4 weeks after injury. The expression of LIMK1 began to fluctuate at 1 week after injury and reached the peak at 3 weeks. Conclusion the expression of GFAP in the spinal cord of rats began to decrease at 4 weeks after injury. The expression of LIMK1 and GFAP was closely related to the injury. Combined with LIMK1 protein expression profile, LIMK1 pathway is involved in the formation and regulation of glial scar.
【作者单位】：河南省安阳地区医院;佳木斯大学第一附属医院;天津医院;
【分类号】：R651.2

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