迷走神经电刺激对脑外伤后昏迷大鼠前额叶皮质去甲肾上腺素α1受体表达变化的影响

发布时间：2018-04-25 17:46

本文选题：迷走神经电刺激 + 脑外伤　；参考：《中国康复医学杂志》2017年01期

【摘要】：目的:探讨迷走神经电刺激(vagus nerve stimulation,VNS)对脑外伤(traumatic brain injury,TBI)后昏迷大鼠的促醒作用及其可能机制。方法:健康成年SD大鼠90只,随机分为空白对照组、假刺激组和刺激组。TBI后昏迷大鼠给予VNS治疗,观察其行为学变化,并通过免疫组织化学和Western-blot技术检测各实验组大鼠前额叶皮质(prefrontal cortex,PFC)组织中去甲肾上腺素α1受体(α1R)含量。结果:假刺激组和刺激组大鼠各30只,观察行为学变化发现,刺激组中20只出现翻正反射,假刺激组仅8只出现翻正反射;将3个实验组中α1R含量进行两两比较,发现刺激组α1R水平均显著高于其他两组(P0.05)。结论:VNS可改善TBI后昏迷大鼠的意识状态水平,对促进TBI后昏迷大鼠的觉醒有积极的治疗作用,其机制可能与PFC去甲肾上腺素α1R水平上调有关。
[Abstract]:Objective: to investigate the effects of vagus nerve stimulation (VNS) on the wake-up of coma rats after traumatic brain injury-induced brain injury (TBI) and its possible mechanism. Methods: 90 healthy adult SD rats were randomly divided into control group, sham stimulation group and stimulative group. The rats in coma group were treated with VNS, and their behavioral changes were observed. The content of norepinephrine 伪 1 receptor (伪 1R) in prefrontal cortex of rats in each experimental group was determined by immunohistochemistry and Western-blot technique. Results: there were 30 rats in the sham stimulation group and 30 rats in the stimulation group. The behavioral changes showed that 20 rats in the stimulation group and 8 rats in the sham stimulation group had positive inversion reflex, and the contents of 伪 1R in the three experimental groups were compared in pairs. It was found that the level of 伪 1R in the stimulation group was significantly higher than that in the other two groups. ConclusionVNS can improve the level of consciousness in coma rats after TBI, and has a positive therapeutic effect on the arousal of coma rats after TBI. The mechanism may be related to the up-regulation of PFC norepinephrine 伪 1R level.
【作者单位】：南昌大学第一附属医院康复医学科;
【基金】：国家自然科学基金资助项目(81260295)
【分类号】：R651.15

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