丙泊酚对鼠胚胎神经干细胞凋亡的影响及作用机制

发布时间：2018-05-03 04:36

本文选题：丙泊酚 + 胚胎神经干细胞　；参考：《广州医科大学学报》2016年04期

【摘要】：目的:探讨丙泊酚对鼠胚胎神经干细胞凋亡的影响及作用机制。方法:选择孕14~16 d Wistar大鼠,分离培养胚胎神经干细胞,分别用5、25、50、100μmol/L丙泊酚进行处理,同时设立脂肪乳剂组和空白对照组。采用MTT法检测细胞存活率;流式细胞术观察丙泊酚对胚胎神经干细胞凋亡的影响;提取对照组、脂肪乳剂组、50μmol/L丙泊酚、50μmol/L丙泊酚+caspase抑制剂培育12 h后的全细胞蛋白,Western-blot检测激活的细胞色素c、caspase-3、caspase-8、caspase-9蛋白的表达。结果:丙泊酚可引起胚胎神经干细胞凋亡,其效应呈剂量依赖型(P0.05);丙泊酚处理后的胚胎神经干细胞激活的caspase-3、CytochromeC、caspase-9、caspase-8蛋白表达水平显著增加(P0.05)。结论:丙泊酚主要通过激活caspase依赖的内源性和外源性凋亡通路引起鼠胚胎神经干细胞凋亡。
[Abstract]:Objective: to investigate the effect and mechanism of propofol on apoptosis of embryonic neural stem cells. Methods: embryonic neural stem cells (ENSCs) were isolated and cultured from Wistar rats on the 16th day of gestation. They were treated with propofol at the concentration of 50 渭 mol/L. The fat emulsion group and the blank control group were set up at the same time. MTT assay was used to detect cell survival rate; flow cytometry was used to observe the effect of propofol on apoptosis of embryonic neural stem cells. In fat emulsion group, 50 渭 mol/L propofol and 50 渭 mol/L propofol caspase inhibitor were cultured for 12 h. The expression of activated cytochrome caspase-3, caspase-8 and caspase-9 was detected by Western-blot. Results: propofol could induce apoptosis of embryonic neural stem cells in a dose-dependent manner, and the expression of caspase-3, cytochrome Caspase-9 and caspase-8 protein in embryonic neural stem cells activated by propofol increased significantly (P 0.05). Conclusion: propofol induces apoptosis of embryonic neural stem cells by activating endogenous and exogenous apoptosis pathways dependent on caspase.
【作者单位】：江西省肿瘤医院麻醉科;
【基金】：江西省自然科学基金(20122BAB215036)
【分类号】：R614

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