鞘内注射右美托咪啶对大鼠急性疼痛行为及脊髓背角c-Fos蛋白表达的影响

发布时间：2018-05-03 06:06

本文选题：急性疼痛 + 右美托咪定　；参考：《南昌大学》2017年硕士论文

【摘要】：目的:观察鞘内注射右美托咪定对切口痛模型大鼠急性疼痛行为学指标的影响,并观察其对脊髓背角c-Fos蛋白表达的影响,探讨其可能的作用机制,为术后疼痛的防治提供有效的临床研究及理论依据。方法:本实验共分为两部分:(1)鞘内注射右美托咪定对切口痛模型大鼠急性疼痛行为学指标的影响。(2)鞘内注射右美托咪定对切口痛模型大鼠血清炎性细胞因子(TNF-α、IL-6和IL-10)及脊髓背角c-Fos蛋白表达的影响。选择健康成年雄性SD大鼠96只,体重250~300g,采用随机数字表法,将其随机分为4组,每组24只。正常对照组(C组):不做任何处理;手术处理组(O组):行右足切开手术;生理盐水+手术处理组(N组):鞘内注射生理盐水20μl后行右足切开手术;右美托咪定+手术处理组(D组):鞘内注射右美托咪定1ug/kg(容量为20μl)后行右足切开手术。经上述处理后,分别于术前(T0)、术后1h、2h、4h、8h、12h、24h及48h时(T1~T7)测定大鼠右足急性疼痛行为学指标[机械缩足反应阈值(MWT)和热缩足潜伏期(TWL)];并分别于上述时点经腹主动脉采集血样,采用Elisa法检测血清TNF-α、IL-6以及IL-10的浓度;采血结束后处死大鼠,灌注4%多聚甲醛,取L4-6脊髓段,采用免疫组织化学法,检测鞘内注射右美托咪定对切口痛模型大鼠脊髓背角c-Fos蛋白表达的影响。结果:(1)与C组比较,O组、N组和D组大鼠术后机械缩足反应阈值(MWT)降低,热缩足潜伏期(TWL)缩短(P0.05);与O组比较,D组大鼠术后机械缩足反应阈值(MWT)升高,热缩足潜伏期(TWL)延长(P0.05),N组差异无统计学意义(P0.05);与N组比较,D组大鼠术后机械缩足反应阈值(MWT)升高,热缩足潜伏期(TWL)延长(P0.05)。(2)与C组比较,O组、N组和D组大鼠术后血清TNF-α、IL-6以及IL-10浓度升高(P0.05);与O组比较,D组大鼠术后血清TNF-α及IL-6浓度降低,IL-10浓度升高(P0.05),N组差异无统计学意义(P0.05);与N组比较,D组大鼠术后血清TNF-α及IL-6浓度降低,IL-10浓度升高(P0.05)。(3)与C组比较,O组、N组和D组大鼠术后脊髓背角c-Fos蛋白表达明显升高(P0.05);与O组比较,D组大鼠脊髓背角c-Fos蛋白表达明显降低(P0.05),N组差异无统计学意义(P0.05);与N组比较,D组大鼠脊髓背角c-Fos蛋白表达明显降低(P0.05)。结论:鞘内预先注射右美托咪定能显著提高切口痛模型大鼠机械痛阈值,减轻大鼠急性疼痛行为,其机制可能与其降低炎性反应及大鼠脊髓背角c-Fos蛋白表达有关。
[Abstract]:Objective: to observe the effect of intrathecal injection of dexmetomidine on the behavioral parameters of acute pain in rats with incision pain, and to observe the effect of dexmetidine on the expression of c-Fos protein in spinal dorsal horn, and to explore its possible mechanism. To provide effective clinical research and theoretical basis for the prevention and treatment of postoperative pain. Methods: this experiment was divided into two parts: (1) Intrathecal injection of dexmetomidine on acute behavioral index of pain induced by incision pain in rats. (2) intrathecal injection of dexmetomidine on serum inflammatory cytokine TNF- 伪 IL-6 and IL-6 in rats with incision pain IL-10) and the expression of c-Fos protein in spinal dorsal horn. 96 male Sprague-Dawley (SD) rats were randomly divided into 4 groups with 24 rats in each group. Normal control group C: no treatment, operation group O: right foot incision, saline operation group: intrathecal injection of normal saline 20 渭 l, right foot incision; Group D: right foot incision was performed after intrathecal injection of dexmetomidine at a dose of 20 渭 l. After the above treatment, the behavioral indexes of acute pain in right foot of rats were measured before operation, 1 h, 2 h, 4 h, 8 h, 12 h, 24 h and 48 h, respectively. Blood samples were collected from abdominal aorta of rats at the same time points, including mechanical foot contraction threshold (MWTs) and thermal contraction latency (TWL). Serum TNF- 伪 IL-6 and IL-10 levels were detected by Elisa method, the rats were killed after blood collection, 4% paraformaldehyde was infused, L4-6 spinal cord segment was taken out, and immunohistochemical method was used. To investigate the effect of intrathecal injection of dexmetomidine on the expression of c-Fos in spinal dorsal horn of rats with incision pain. Results compared with group C, the MWTs of rats in group O and group D were decreased, and the latency of thermal contraction was shortened (P 0.05), and the threshold of mechanical contraction in group D was higher than that in group O. There was no significant difference between group N and group N in prolonging the latent period of thermal contraction (TWL), and the threshold of mechanical foot contraction in group D was higher than that in group N. Compared with group C, the levels of TNF- 伪 IL-6 and IL-10 were increased in group N and group D, but there was no significant difference between group D and group D in decreasing serum concentration of TNF- 伪 and IL-6 and increasing concentration of IL-10. Compared with group N and group C, the expression of c-Fos protein in spinal dorsal horn of rats in group D and group D was significantly higher than that in group C and group O, respectively, and the expression of c-Fos in group D was significantly higher than that in group O, compared with group O, the expression of c-Fos in the spinal cord of group D was significantly higher than that in group D, and that in group D was significantly higher than that in group C (P 0.05), and the expression of c-Fos in group D was significantly higher than that in group C (P 0.05). There was no significant difference in the expression of c-Fos protein between the two groups, and the expression of c-Fos protein in the spinal dorsal horn of the D group was significantly lower than that of the N group. Conclusion: intrathecal injection of dexmetidine can significantly increase the mechanical pain threshold and alleviate the acute pain behavior of rats with incision pain. The mechanism may be related to the decrease of inflammatory response and the expression of c-Fos protein in the dorsal horn of spinal cord in rats.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R614

【参考文献】