MAGEA在骨肉瘤中的表达和预后分析

发布时间：2018-05-10 10:56

本文选题：骨肉瘤 + 基因芯片　；参考：《实用医学杂志》2016年16期

【摘要】：目的:研究恶性黑色素瘤抗原基因A(MAGEA)在骨肉瘤中的表达特点及与骨肉瘤预后的相关性。方法:利用基因芯片方法筛选在骨肉瘤中特异性高表达基因。利用Realtime PCR和Western-blot的方法验证其在骨肉瘤细胞和组织中的表达。用免疫组化方法研究其在骨肉瘤组织中的表达,分析其与预后的关系。结果:MAGEA家族基因在骨肉瘤中协同高表达,在正常成骨细胞中无表达。MAGEA在肺转移灶中的表达水平明显高于原发灶。MAGEA表达阳性的骨肉瘤患者肺转移风险升高(RR=2.79,95%CI,1.12-6.93,P=0.028),预后较差(80%±8.9%vs 39.6%±8.4%,P=0.01)结论 :MAGEA家族基因在骨肉瘤组织中的高表达,是骨肉瘤预后的重要标志。
[Abstract]:Objective: to study the expression of malignant melanoma antigen gene Agna in osteosarcoma and its correlation with the prognosis of osteosarcoma. Methods: specific overexpression genes in osteosarcoma were screened by gene chip method. Realtime PCR and Western-blot were used to detect its expression in osteosarcoma cells and tissues. Immunohistochemical method was used to study the expression of osteosarcoma and its relationship with prognosis. Results the member MAGEA family gene was co-expressed in osteosarcoma. The expression level of MAGEA in normal osteoblasts was significantly higher than that in patients with primary osteosarcoma with positive expression of .MAGEA. The risk of lung metastasis in osteosarcoma patients was significantly higher than that in patients with osteosarcoma with positive expression of .MAGEA. The risk of lung metastasis in patients with osteosarcoma was significantly higher than that in patients with primary osteosarcoma. The risk of lung metastasis in osteosarcoma patients was significantly higher than that in patients with osteosarcoma with positive expression of .MAGEA. It is an important prognostic marker of osteosarcoma.
【作者单位】：中山大学附属第一医院骨肿瘤科;南方医科大学第三附属医院口腔科;中山大学附属第一医院病理科;
【基金】：国家自然科学基金青年项目(编号:81001194) 教育部高等院校博士学科点专项基金(编号:20100171120077)
【分类号】：R738.1

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