SOST和β-catenin在不同分期膝骨关节炎患者软骨及软骨下骨表达的研究

发布时间：2018-05-14 12:46

本文选题：骨关节炎 + 软骨　；参考：《中国骨质疏松杂志》2016年06期

【摘要】：目的通过测定β-catenin和骨硬化蛋白(SOST)在不同分期人膝关节骨关节炎软骨、软骨下骨中的表达变化情况,探讨Wnt-β-catenin信号通路在骨关节炎患者的疾病进展中起到的作用。方法所有的骨关节炎标本均取自因膝关节骨关节炎合并内翻屈曲畸形行全膝人工关节置换术(TKA)的患者自愿捐赠的新鲜内侧胫骨平台组织,共39例。正常的软骨及软骨下骨组织均取自因下肢毁损伤行下肢截肢术的患者所捐赠的新鲜内侧胫骨平台组织,共6例。切片后用番红O固绿染色,采用改良Mankin评分将所有标本分为正常组,中度OA组和重度OA组。用免疫组化方法检测这3组中软骨及软骨下骨中β-catenin和SOST的表达情况。结果根据改良Mankin评分结果,45例标本中,6例为正常,14例为中度OA,25例为重度OA。β-catenin在软骨和软骨下骨中表达均持续升高,软骨中染色阳性细胞数量百分比在正常组,中度OA组和重度OA组中分别为17%,49%和62%,软骨下骨中分别为42%,59%和74%。而软骨中SOST染色阳性细胞百分比在则在中度OA组中表达量最高(59%),在正常组(11%)和重度OA组(12%)中表达量均明显少于中度组。在软骨下骨中,SOST染色阳性细胞百分比正常组中为58%,中度OA组为38%,重度OA组为16%,其表达量持续降低。结论β-catenin及SOST与骨关节炎疾病的发展及严重程度有关,甚至影响到Wnt-β-catenin信号通路,其可能在骨关节炎疾病的发展中起到重要作用。
[Abstract]:Objective to investigate the role of Wnt- 尾 -catenin signaling pathway in the progression of osteoarthritis by measuring the expression of 尾 -catenin and osteosclerotic protein (SOST) in cartilage and subchondral bone of knee osteoarthritis at different stages. Methods all the specimens of osteoarthritis were obtained from the fresh medial tibial plateau of 39 patients with knee osteoarthritis combined with varus flexion deformity. The normal cartilage and subchondral bone tissue were obtained from fresh medial tibial plateau tissue of 6 cases. All specimens were divided into normal group, moderate OA group and severe OA group by modified Mankin score. The expression of 尾 -catenin and SOST in cartilage and subchondral bone were detected by immunohistochemical method. Results according to the modified Mankin score, the expression of OA. 尾 -catenin in cartilage and subchondral bone continued to increase, and the percentage of staining positive cells in cartilage was higher in normal group than in normal group, and 6 cases were normal, 14 cases were moderate, 25 cases were severe OA. 尾 -catenin expression in cartilage and subchondral bone. In moderate OA group and severe OA group, 17% and 62%, 422% and 74% in subchondral bone, respectively. However, the percentage of SOST staining positive cells in cartilage was significantly lower in moderate OA group than in moderate OA group, and was significantly lower in normal group and severe OA group than in moderate OA group. The percentage of positive cells in subchondral bone was 58 in normal group, 38 in moderate OA group and 16 in severe OA group. Conclusion 尾 -catenin and SOST are related to the development and severity of osteoarthritis, and even affect Wnt- 尾 -catenin signaling pathway, which may play an important role in the development of osteoarthritis.
【作者单位】：宁夏医科大学总医院骨三科;
【分类号】：R684.3

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