丙泊酚对肾缺血-再灌注损伤大鼠肾叶间动脉缝隙连接Cx43表达的影响

发布时间：2018-06-09 04:12

  本文选题：丙泊酚 + 肾缺血-再灌注损伤　； 参考：《西安交通大学学报(医学版)》2017年05期

【摘要】：目的观察丙泊酚干预对肾缺血再灌注损伤大鼠肾叶间动脉(RIA)Cx43表达的影响。方法雄性SD大鼠随机分为空白对照(control)4h、24h组,假手术(sham)4h、24h组,肾缺血再灌注(I/R)4h、24h组,丙泊酚干预(propofol)4h、24h组,脂肪乳剂(intralipid)4h、24h组。采用切除右肾、无创动脉夹夹闭左肾制备肾缺血再灌注模型,肾缺血45min,按分组不同分别再灌注4h、24h;采用全自动生化仪检测血清尿素氮(serum urea nitrogen,BUN)和肌酐(creatinine,Cr);HE染色法观察肾组织的病理学变化;压力肌动图技术观察RIA收缩舒张的变化;Western blot方法检测RIA Cx43蛋白的表达。结果与空白对照组相比,假手术组血清BUN、Cr浓度无统计学差异,肾HE染色未发现明显的形态学改变,压力肌动图观察RIA收缩率无统计学差异,Cx43蛋白表达无统计学差异;与假手术组相比,肾缺血再灌注组血清BUN、Cr浓度均显著增高,肾HE切片显示肾组织病变明显,压力肌动图显示RIA收缩率下降,Cx43蛋白表达显著增高,差异有统计学差异(P0.05);与肾缺血再灌注组相比,丙泊酚干预组血清BUN、Cr浓度有所降低,肾HE切片显示肾组织病变减轻,RIA收缩率有所升高,Cx43蛋白表达降低,差异有统计学意义(P0.05)。结论丙泊酚可能通过降低RIA Cx43蛋白的表达改变血管舒缩性,从而减轻肾缺血再灌注损伤。
[Abstract]:Objective to observe the effect of propofol on the expression of RIA-Cx43 in renal interlobar artery during renal ischemia-reperfusion injury in rats. Methods male Sprague-Dawley rats were randomly divided into control group (control group), sham group (n = 4), renal ischemia reperfusion group (n = 4), propofol intervention group (n = 24) and fat emulsion intra (n = 4 h) group (n = 24). The model of renal ischemia-reperfusion was established by resection of right kidney and occlusion of left kidney by non-invasive artery clamp. After 45 minutes of renal ischemia, the rats were divided into different groups for 4 hours and 24 hours after reperfusion respectively. The pathological changes of renal tissues were observed by using automatic biochemical instrument to detect serum urea nitrogenbun and creatinine (creatinine). The changes of systolic and diastolic activity of RIA were observed by pressure-actin technique. The expression of RIA Cx43 protein was detected by Western blot. Results compared with the blank control group, there was no significant difference in serum BUNN Cr concentration, no obvious morphological changes in renal HE staining, and no significant difference in the expression of Cx43 protein in the RIA contraction rate observed by pressure-actigraphy. Compared with the sham-operated group, the serum BUNN Cr concentration in the renal ischemia reperfusion group was significantly higher, the renal HE section showed obvious renal tissue lesion, and the pressure actin graph showed that the RIA contraction rate decreased and the expression of Cx43 protein increased significantly. Compared with the renal ischemia-reperfusion group, the serum BUNX Cr concentration in the propofol treated group was decreased, and the renal HE section showed that the renal tissue lesion was alleviated and the RIA contraction rate was increased, the expression of Cx43 protein was decreased, and the difference was statistically significant (P 0.05). Conclusion propofol may reduce renal ischemia-reperfusion injury by decreasing the expression of RIA Cx43 protein.
【作者单位】： 石河子大学医学院生理教研室/新疆地方与民族高发病教育部重点实验室;
【基金】：国家自然科学基金资助项目(No.81560081)~~
【分类号】：R614
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本文编号：1998836