盐酸戊乙奎醚在大鼠急性重症胰腺炎相关肺损伤中的作用

发布时间：2018-06-18 07:57

本文选题：盐酸戊乙奎醚 + 重症急性胰腺炎　；参考：《临床麻醉学杂志》2017年09期

【摘要】：目的探讨盐酸戊乙奎醚在大鼠急性重症胰腺炎相关肺损伤(PALI)中的作用及对缺氧诱导因子(HIF)-1α相关炎症因子表达的影响。方法 40只健康雄性成年SD大鼠,应用随机数字法分为三组:假手术组(S组,n=8)、PALI模型组(ALI组,n=16)、盐酸戊乙奎醚+PALI模型组(P组,n=16)。ALI组和P组采用4%牛磺胆酸钠1ml/kg逆行性胰胆管注射法建立大鼠PALI模型。P组在建立模型后即刻给予大鼠腹腔内注射盐酸戊乙奎醚,S组和ALI组经腹腔注射等量生理盐水。造模后12h处死大鼠,取肺组织称重后计算肺湿干重比(W/D),光镜下观察胰腺及肺组织病理学评分,ELISA法测定肺组织HIF-1α、IL-1β、IL-6蛋白含量和血清胰淀粉酶含量,Western blot检测肺组织TLR4、NF-κB p65蛋白含量。结果 ALI组和P组大鼠的胰腺组织均存在广泛的中性粒细胞浸润、腺泡出血坏死和脂肪坏死。与S组比较,ALI组与P组胰腺病理评分、肺损伤评分、肺W/D明显升高,胰淀粉酶含量明显降低(P0.01);与ALI组比较,P组肺损伤评分、肺W/D明显降低(P0.05)。与S组比较,ALI组和P组大鼠肺组织HIF-1α、IL-1β、IL-6含量明显升高,肺组织TLR4、NF-κB p65含量明显升高(P0.01);与ALI组比较,P组肺组织HIF-1α、IL-1β、IL-6含量明显降低,TLR4、NF-κB p65蛋白含量明显降低(P0.05)。结论盐酸戊乙奎醚在不改变急性重症胰腺炎的胰腺组织损伤的情况下能够显著减轻急性重症胰腺炎相关肺损伤,机制可能与抑制HIF-1α等相关炎性因子的表达相关。
[Abstract]:Objective to investigate the effect of pentehyclidine hydrochloride on acute severe pancreatitis associated lung injury (PALI) and the expression of hypoxia inducible factor (HIF-1 伪) -associated inflammatory factor (HIF-1 伪) in rats. Methods 40 healthy male adult SD rats were used. The rats were divided into three groups by random number method: sham operation group (S group), palli group (model group), palli group (P group) and P group (group P) were injected with 4% sodium taurocholate (1ml/kg) to establish the model of PALI in rats. P group was given retrograde injection of sodium taurocholate into the pancreatopancreatopancreatine cholangiopancreatine (PALI) group. The model of PALI in rats was established by injecting 4% sodium taurocholate into the pancreatic duct. The rats were injected intraperitoneally with pentanethaquine hydrochloride S group and Ali group by intraperitoneal injection of the same amount of normal saline immediately after the establishment of the model. The rats were killed 12 hours after the model. The lung wet / dry weight ratio was calculated after weighing the lung tissue. The histopathological scores of pancreas and lung were observed under light microscope. The contents of HIF-1 伪, IL-1 尾 and IL-6 in lung tissue and the content of serum amylase in lung tissue were measured by Elisa. The protein content of TLR4NF- 魏 B p65 in lung tissue was detected by Western blot. Results there were extensive neutrophil infiltration, acinar hemorrhage necrosis and fat necrosis in pancreatic tissues of Ali and P groups. Compared with group S, the pancreatic pathological score, lung injury score, lung W / D and amylopsin content in Ali group and Ali group were significantly increased, and the lung injury score in P group was significantly lower than that in Ali group. Compared with group S, the contents of HIF-1 伪 -IL-1 尾 and IL-6 in lung tissue of rats in Ali and P groups were significantly increased, while the content of NF- 魏 B p65 in lung tissue of TLR4NF-kappa B was significantly higher than that in group S, and the content of HIF-1 伪 -IL-1- 尾 -guan-6 in lung tissue of group P was significantly lower than that of group Ali, and the content of protein of TLR4NF- 魏 B p65 was significantly decreased in group A (P 0.05). Conclusion Penehyclidine hydrochloride can significantly reduce the lung injury associated with severe acute pancreatitis without changing the pancreatic tissue injury of severe acute pancreatitis. The mechanism may be related to the inhibition of the expression of HIF-1 伪 and other related inflammatory factors.
【作者单位】：郑州颐和医院麻醉科;郑州大学第一附属医院肝胆胰外科;
【基金】：国家自然科学基金(81301618)
【分类号】：R614

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