谷甾醇-3-O-葡萄糖苷对瘢痕疙瘩成纤维细胞增殖、凋亡及胶原合成的影响

发布时间：2018-06-20 03:58

本文选题：谷甾醇--O-葡萄糖苷 + 瘢痕疙瘩成纤维细胞　；参考：《中国比较医学杂志》2017年12期

【摘要】：目的探索谷甾醇-3-O-葡萄糖苷对瘢痕疙瘩成纤维细胞(keloid fibroblasts,KF)抑制作用的研究。方法 0(对照组),3.125、6.25、12.5、25、50、100μg/m L的谷甾醇-3-O-葡萄糖苷作用KF细胞48 h后,MTT法检测细胞活力,Ed U染色法检测细胞增殖,流式细胞术分别检测细胞凋亡及细胞周期,酶联免疫吸附试验(ELISA)检测细胞中COL I、COL III含量。结果 3.125、6.25、12.5、25、50、100μg/m L谷甾醇-3-O-葡萄糖苷能显著的降低KF细胞活力(P0.01),提高细胞增殖抑制率(P0.01),IC50=(27.54±2.18)μg/m L。与对照组比较,12.5、25、50μg/m L谷甾醇-3-O-葡萄糖苷能显著的降低细胞增殖率(P0.01),提高细胞早期及晚期凋亡率(P0.01),使细胞周期阻滞在G1期(P0.01),同时还能显著的降低细胞中COL I、COL III含量(P0.01)。结论谷甾醇-3-O-葡萄糖苷能显著的抑制KF细胞增殖,诱导细胞凋亡,同时还能抑制胶原的合成。
[Abstract]:Objective to investigate the inhibitory effect of sitosterol-3-O- glucoside on keloid fibroblast KF in keloid fibroblasts. Methods the KF cells were treated with sitosterol -3-O- glucoside for 48 h. The proliferation of KF cells was detected by MTT assay. The apoptosis and cell cycle of KF cells were detected by flow cytometry. Enzyme linked immunosorbent assay (Elisa) was used to detect the content of Col III in cells. Results 3.125 ~ 6.25 ~ (12.5) 渭 g / mL sitosterol -3-O- glucoside could significantly reduce the activity of KF cells and increase the cell proliferation inhibition rate (P _ (0.01) ~ (50) 渭 g / m ~ (-1) ~ (27. 54 卤2. 18) 渭 g / m ~ (-1). Compared with the control group, 12.5g / mL sitosterol -3-Oglucoside could significantly decrease the cell proliferation rate and increase the cell apoptosis rate in the early and late stages of the cell, and make the cell cycle arrest in G1 phase (P0.01A). At the same time, it could significantly reduce the content of Col ICOL III in the cells. Conclusion sitosterol-3-O- glucoside can significantly inhibit the proliferation of KF cells, induce apoptosis and inhibit the synthesis of collagen.
【作者单位】：白城医学高等专科学校;大连医科大学附属第一医院;
【基金】：吉林省教育厅“十二五”科学技术研究规划项目(吉教科合字[2014]第451号)
【分类号】：R622

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