不同缺血后调适方案对大鼠肢体骨骼肌缺血再灌注损伤影响的实验研究
发布时间：2018-10-25 09:58
【摘要】：目的：建立SD大鼠肢体骨骼肌缺血再灌注损伤的模型,研究不同缺血后调适方案对大鼠肢体骨骼肌缺血再灌注损伤是否具有保护作用并优选最佳方案,为进一步研究提供基础；并初步探讨缺血后调适在肢体骨骼肌缺血再灌注损伤中的可能机制。方法：健康成年雄性SD大鼠随机分为4组(n=9)：缺血再灌注组(IRI组)和不同缺血后调适3个组(IpostA、IpostB、IpostC组)。各组大鼠均在右侧股动脉进行缺血再灌注实验操作,并分离左侧股动脉作为自身假手术对照,其中缺血再灌注组给予右侧股动脉缺血4h再灌注24h；缺血后调适3个组(IpostA、IpostB、IpostC组)分别于右侧4h缺血后立即施加4个循环分别为10s再灌/10s缺血、30s再灌/30s缺血、1min再灌/lmin缺血的后调适操作,再灌注24h。各组实验结束后抽血检测乳酸脱氢酶(LDH),取样腓肠肌测算湿干重(湿/干,W/D)比值、检测髓过氧化物酶(MPO)和丙二醛(MDA),取样胫前肌电镜观察骨骼肌病理变化。结果：IRI组实验侧W/D值较假手术侧明显增加(P0.05)；4个循环的30s再灌/30s缺血后调适IpostB组W/D值显著低于IRI组(P0.05),其余2个缺血后调适组(IpostA、IpostC组)与IRI组差异不明显(P0.05)。LDH值、MPO值、MDA值,缺血后调适3组均低于IRI组(P0.05),缺血后调适组间未见明显差异。4个循环的30s再灌/30s缺血IpostB组电镜下骨骼肌线粒体嵴的空泡变性程度、肌原纤维结构清晰程度和细胞核完整性较缺血再灌注组均有明显改善,其余2个缺血后调适组超微结构较缺血再灌注组也有不同程度改善。结论：1、缺血后调适对大鼠肢体骨骼肌缺血再灌注损伤有保护作用,4个循环30s再灌/30s缺血方案的保护效果最明显,可作为进一步实验研究的基础。2、缺血后调适在骨骼肌缺血再灌注损伤中发挥保护作用可能的机制是通过减少活性氧类物质的生成、减轻肌纤维水肿、中性白细胞聚集、能量代谢等发挥作用的。
[Abstract]:Objective: to establish a model of limb skeletal muscle ischemia-reperfusion injury in SD rats, and to study whether different ischemic conditioning schemes have protective effect on limb skeletal muscle ischemia-reperfusion injury and to select the best scheme for further study. To explore the possible mechanism of ischemic conditioning in limb skeletal muscle ischemia reperfusion injury. Methods: healthy adult male SD rats were randomly divided into 4 groups: ischemia reperfusion group (IRI group) and different ischemic conditioning group (IpostA,IpostB,IpostC group). All the rats in each group were subjected to ischemia-reperfusion experiment in the right femoral artery, and left femoral artery was separated as self-sham-operation control. The ischemia-reperfusion group was given right femoral artery ischemia reperfusion for 4 h and 24 h. Three groups (IpostA,IpostB,IpostC group) were subjected to 10 s reperfusion / 10 s ischemia, 30s reperfusion / 30s ischemia, 1min reperfusion / lmin ischemia and 24 h reperfusion immediately after right 4 h ischemia. After the experiment, lactate dehydrogenase (LDH),) was taken from gastrocnemius muscle to calculate wet dry weight (wet / dry, W / D) ratio, myeloperoxidase (MPO) and malondialdehyde (MDA),) were taken from tibial muscle to observe the pathological changes of skeletal muscle. Results: the W / D value of experimental side in IRI group was significantly higher than that in sham operation side (P0.05), the WR value in IpostB group after 30 s reperfusion / 30 s ischemia was significantly lower than that in IRI group (P0.05), there was no significant difference between the other two ischemic conditioning groups (IpostA,IpostC group) and IRI group (P0.05). LDH, MPO value, MDA value). The degree of vacuolar degeneration of mitochondrial crest of skeletal muscle was observed under electron microscope in 4 circulations of 30 s reperfusion / 30 s ischemia IpostB group, which was lower than that in IRI group (P0.05), and there was no significant difference between the three groups (P0.05). The structural clarity and nuclear integrity of myofibrils were improved significantly compared with those of ischemia reperfusion group, and the ultrastructure of the other two ischemic conditioning groups was improved to some extent than that of ischemia reperfusion group. Conclusion: 1.After-ischemic conditioning has protective effect on limb skeletal muscle ischemia-reperfusion injury, and the protective effect of four cycles for 30s reperfusion / 30s ischemia is the most obvious. 2. The possible protective mechanism of ischemic conditioning in skeletal muscle ischemia-reperfusion injury is to reduce muscle fiber edema and neutrophil aggregation by reducing the production of reactive oxygen species (Ros). Functioning in energy metabolism, etc.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R687
本文编号：2293364
[Abstract]:Objective: to establish a model of limb skeletal muscle ischemia-reperfusion injury in SD rats, and to study whether different ischemic conditioning schemes have protective effect on limb skeletal muscle ischemia-reperfusion injury and to select the best scheme for further study. To explore the possible mechanism of ischemic conditioning in limb skeletal muscle ischemia reperfusion injury. Methods: healthy adult male SD rats were randomly divided into 4 groups: ischemia reperfusion group (IRI group) and different ischemic conditioning group (IpostA,IpostB,IpostC group). All the rats in each group were subjected to ischemia-reperfusion experiment in the right femoral artery, and left femoral artery was separated as self-sham-operation control. The ischemia-reperfusion group was given right femoral artery ischemia reperfusion for 4 h and 24 h. Three groups (IpostA,IpostB,IpostC group) were subjected to 10 s reperfusion / 10 s ischemia, 30s reperfusion / 30s ischemia, 1min reperfusion / lmin ischemia and 24 h reperfusion immediately after right 4 h ischemia. After the experiment, lactate dehydrogenase (LDH),) was taken from gastrocnemius muscle to calculate wet dry weight (wet / dry, W / D) ratio, myeloperoxidase (MPO) and malondialdehyde (MDA),) were taken from tibial muscle to observe the pathological changes of skeletal muscle. Results: the W / D value of experimental side in IRI group was significantly higher than that in sham operation side (P0.05), the WR value in IpostB group after 30 s reperfusion / 30 s ischemia was significantly lower than that in IRI group (P0.05), there was no significant difference between the other two ischemic conditioning groups (IpostA,IpostC group) and IRI group (P0.05). LDH, MPO value, MDA value). The degree of vacuolar degeneration of mitochondrial crest of skeletal muscle was observed under electron microscope in 4 circulations of 30 s reperfusion / 30 s ischemia IpostB group, which was lower than that in IRI group (P0.05), and there was no significant difference between the three groups (P0.05). The structural clarity and nuclear integrity of myofibrils were improved significantly compared with those of ischemia reperfusion group, and the ultrastructure of the other two ischemic conditioning groups was improved to some extent than that of ischemia reperfusion group. Conclusion: 1.After-ischemic conditioning has protective effect on limb skeletal muscle ischemia-reperfusion injury, and the protective effect of four cycles for 30s reperfusion / 30s ischemia is the most obvious. 2. The possible protective mechanism of ischemic conditioning in skeletal muscle ischemia-reperfusion injury is to reduce muscle fiber edema and neutrophil aggregation by reducing the production of reactive oxygen species (Ros). Functioning in energy metabolism, etc.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R687
【参考文献】
相关期刊论文 前1条
1 孙备;细胞凋亡与缺血-再灌注损伤的研究进展[J];国外医学.外科学分册;1998年06期
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