H-型血管在骨关节炎软骨下骨中的表达及作用

发布时间：2019-02-15 03:03

【摘要】：背景:H-型血管主要分布在骨骼干骺端,能促进骨量增多,加速骨的形成。目的:观察骨关节炎发生发展过程中软骨下骨H-型血管的表达情况。方法:8周龄健康清洁级C57小鼠随机分为实验组和假手术组。实验组小鼠行右侧膝关节内侧半月板切除诱导骨关节炎(MMx模型);假手术组小鼠则仅切开关节囊而不行内侧半月板切除。术后4周取膝关节标本进行组织学分析,番红O快绿染色评估软骨损伤程度。免疫荧光检测CD31、Emcn、基质金属蛋白酶13表达情况,苏木精-伊红染色观察软骨下骨改变情况,Micro CT分析软骨下骨骨量的变化。结果与结论:(1)与假手术组比较,实验组OARSI评分、软骨下骨CD31、Emcn共荧光、H-型血管、基质金属蛋白酶13荧光表达及软骨下骨骨量均显著升高(P0.05);(2)结果说明,骨关节炎过程中,软骨下骨的H-型血管增多,促进软骨下骨增生及重塑。
[Abstract]:Background: h-type blood vessels are mainly distributed in metaphyseal bone, which can promote bone mass and accelerate bone formation. Objective: to observe the expression of H-type vessels in subchondral bone during the development of osteoarthritis. Methods: 8 weeks old healthy C57 mice were randomly divided into experimental group and sham operation group. The right medial meniscal excision induced osteoarthritis (MMx model) was performed in the experimental group while in the sham-operated group the capsule of the joint was cut but not the medial meniscus was removed. Histological analysis was performed 4 weeks after operation, and the degree of cartilage injury was evaluated by fast green staining. The expression of CD31,Emcn, matrix metalloproteinase 13 was detected by immunofluorescence and the changes of subchondral bone were observed by hematoxylin and eosin staining. The changes of bone mass in subchondral bone were analyzed by, Micro CT. Results and conclusion: (1) compared with sham operation group, OARSI score, CD31,Emcn co-fluorescence, H- type blood vessel, matrix metalloproteinase-13 fluorescence expression and bone mass of subchondral bone in the experimental group were significantly higher than those in the sham operation group (P0.05). (2) the results showed that the H-type vessels of subchondral bone increased during osteoarthritis, and promoted the proliferation and remodeling of subchondral bone.
【作者单位】：南方医科大学;南方医科大学第三附属医院广东省骨科研究院;
【基金】：国家自然科学基金(81371990);国家自然科学基金(81601945) 高等学校博士学科点专项科研基金(20124433110021) 广东省医学科研基金(A2015422)~~
【分类号】：R684.3

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