心脏脂联素受体在自发性高血压大鼠模拟左室舒张功能不全进展中的分布特征

发布时间：2018-01-04 21:39

本文关键词：心脏脂联素受体在自发性高血压大鼠模拟左室舒张功能不全进展中的分布特征　出处：《川北医学院》2016年硕士论文　论文类型：学位论文

【摘要】：研究背景:射血分数保留的心力衰竭(heart failure with preserved ejection fraction,HFpEF)是一近年来备受关注的心衰类型。在HFpEF的发病机制中,心脏舒张功能不全(cardiac diastolic dysfunction)发挥重要作用。近来发现舒张功能不全的发生与脂联素(adiponectin,APN)水平降低密切相关。脂联素是脂肪细胞分泌最多的细胞因子,通过脂联素受体(adiponectin receptor 1/2,AdipoR1/2;T-cadherin,T-cad)发挥心脏保护作用,然而,不同脂联素受体在心脏舒张功能不全中的分布特征尚不清楚。目的:观察不同脂联素受体在自发性高血压大鼠(spontaneously hypertensive rat,SHR)模拟舒张功能不全进程中的表达水平。方法:24只雄性SHR大鼠及同月龄的Wistar大鼠,以3个月为时间点,从3月龄起连续的、动态的观察至12月龄。测定尾动脉血压及心率,超声心动图评价心脏结构和功能,HE和Masson染色分别评估心肌肥大和纤维化程度,ELISA检测血清NT-proBNP和脂联素水平以及Western blotting法检测心脏脂联素受体表达。结果:与同月龄Wistar大鼠比较,SHR在6月龄出现舒张功能异常,即E/A比值降低(2.01±0.22vs2.74±0.15,P0,01)、E'/A'比值降低(0.95±0.26vs 1.35±0.32,P0,01)、E/E'比值增加(28.41±1.76 vs 20.66±2.37,P0,01)和血清NT-proBNP水平升高(2.491±0.22ng/ml vs 1.969±0.59ng/ml,p0,01)。心肌及血清脂联素水平均随月龄增加表达量逐渐下调。在3月龄-9月龄间,心肌AdipoR1水平没变化,12月龄开始下降。T-cad于6月龄时下调,并持续该水平至12月龄。然而,AdipoR2在整个观察阶段都保持同一水平。结论:不同脂联素受体在心脏舒张功能不全进展中呈现不同的表达变化,其中,T-cad在心脏舒张功能不全出现时即下调,AdipoR1在严重舒张功能不全时才开始下调,然而,AdipoR2在整个疾病进程中均保持同一水平。
[Abstract]:Background: heart failure with preserved ejection fraction (heart failure with preserved ejection fraction, HFpEF) is a type of heart failure has attracted much attention in recent years. In the pathogenesis of HFpEF, diastolic heart failure (cardiac diastolic dysfunction) play an important role. Recently found that diastolic dysfunction occurs with adiponectin (adiponectin APN) reduced levels of adiponectin is closely related to the secretion of cytokines. Most of the fat cells, the adiponectin receptor (adiponectin receptor, 1/2, T-cadherin, AdipoR1/2; T-cad) cardioprotective effects, however, the different distribution of adiponectin receptors in cardiac diastolic insufficiency is unclear. Objective: To observe the effect of adiponectin receptors in spontaneous hypertension rat (spontaneously hypertensive rat, SHR) simulation expression of diastolic dysfunction in the process. Methods: 24 male SHR rats Wistar rats and the same age, in 3 months time, from March at the age of continuous and dynamic observation to December. Age determination of heart rate and blood pressure of tail artery, echocardiographic heart function and structure evaluation, HE and Masson staining respectively to evaluate myocardial hypertrophy and fibrosis, the serum NT-proBNP and ELISA detection adiponectin and Western blotting method to detect the expression of cardiac adiponectin receptor. Results: compared with age-matched Wistar rats, SHR in June age appeared abnormal diastolic function, which is the ratio of E/A decreased (2.01 + 0.22vs2.74 + 0.15, P0,01), the ratio of E'/A'decreased (0.95 + 0.26vs 1.35 + 0.32, P0,01), the ratio of E/E' increased (28.41 20.66 + 1.76 vs + 2.37, P0,01) and elevated serum NT-proBNP level (2.491 + 0.22ng/ml vs 1.969 + 0.59ng/ml, p0,01). The myocardial and serum adiponectin levels were increased with age was gradually reduced. In March at the age of -9 months, myocardial AdipoR1 level No change, December age began to decline.T-cad in June at the age of decline, and continued to December the level of age. However, AdipoR2 remained the same level in the whole observation period. Conclusion: the different adiponectin receptors in cardiac diastolic dysfunction in showing different expression, T-cad in cardiac diastolic dysfunction occurs when lowered, AdipoR1 in severe diastolic dysfunction began to decline, however, AdipoR2 remained the same level in the disease process.

【学位授予单位】：川北医学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R544.1

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