NPR3基因多态性与中国汉族人群原发性高血压的关系

发布时间：2018-01-25 12:19

本文关键词： 原发性高血压利钠肽清除受体基因单核苷酸多态性体重指数　出处：《宁波大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的探讨利钠肽清除受体(natriuretic peptide clearance receptor,NPRC)基因(NPR3)多态性与原发性高血压(essential hypertension,EH)的关系。方法本研按照病例-对照方法设计,收集原发性高血压患者组452例和年龄、性别相仿的血压正常者为对照组434例作为研究对象。对吸烟史、饮酒史、2型糖尿病病史、高血压病病史和家族史等进行记录。检测血生化项目:包括总胆固醇、甘油三酯、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、空腹血糖、尿酸、肌酐、尿素氮、钠离子和钾离子等指标。使用标签SNP(Tag SNP)策略选择9个NPR3基因SNPs位点(rs3792758、rs1173773、rs13436831、rs11750438、rs1173747、rs2292026、rs976576、rs696831、rs3811953)。采用多聚酶链反应(polymerase chain reaction,PCR)技术扩增NPR3基因片段DNA,经多重SNa Pshot平台技术SNPs基因高效扩增,利用ABI 3130基因分析仪分析基因型,运用Peak Scanner 1.1软件分析结果。NPR3基因SNPs位点基因型在病例和对照组中的分布频率使用Hardy-Weinberg平衡检验。统计软件为SPSS18.0软件,t检验、卡方检验和二元Logistic回归分析用于数据统计分析,双侧检验,P0.05为具有统计学意义。应用Haploview4.2程序作连锁不平衡分析。结果Hardy-Weinberg平衡检验采用卡方检验,所选9个SNPs在病例组和对照组人群中P值均0.05,符合Hardy-Weinberg平衡定律。病例组与对照组NPR3基因SNPs基因型经二元Logistic回归分析,rs1173773GG基因型携带者患高血压病的风险增加,OR值95%CI 3.164(1.011-9.903),P值0.037,具有统计学意义,经调整年龄、性别、体重指数、腰围、吸烟史、饮酒史和高血压病家族史后,OR值95%CI 3.012(1.002-9.728),P值0.041,仍具有统计学意义。其他8个SNPs在两组之间比较未发现患高血压病风险,P值均0.05。两组等位基因型比较经卡方检验,P值均0.05,差异无统计学意义。进一步在高血压病组内比较,BMI"g25Kg/m2与BMI25Kg/m2相比,GG基因型携带者超重或者肥胖的风险更高,OR值95%CI3.509(0.935-13.176),P值0.049,具有统计学意义;G/A等位基因型携带比较,P0.05,无统计学意义。另外,在高血压病组内是否合并2型糖尿病各113名比较,G/A等位基因型携带分布差异无统计学意义。结论本研究发现在中国汉族人群中,NPR3基因rs1173773 GG基因携带者患原发性高血压风险增加;且原发性高血压合并超重或肥胖的风险亦增加。
[Abstract]:Objective to investigate natriuretic peptide clearance receptor. NPRC3) polymorphism was associated with essential hypertensionin essential hypertension (EH). Methods the study was designed with a case-control method. A total of 452 patients with essential hypertension and patients with normal blood pressure of similar age and sex were collected as control group 434 cases as control group. The history of smoking and drinking were compared with the history of type 2 diabetes mellitus. The history of hypertension and family history were recorded. The blood biochemical items included total cholesterol, triglyceride, low density lipoprotein cholesterol, high density lipoprotein cholesterol, fasting blood glucose, uric acid, creatinine. Urea nitrogen, sodium ion and potassium ion were used to select 9 SNPs loci of NPR3 gene, rs3792758 and rs1173773. Rs13436831,rs11750438,rs1173747,rs2292026,rs976576,rs696831. NPR3 gene fragment DNA was amplified by polymerase chain reaction (PCR) and polymerase chain reaction- PCR (PCR) technique. The SNPs gene was amplified efficiently by multiplex SNa Pshot platform, and genotypes were analyzed by ABI 3130 gene analyzer. Using Peak Scanner. 1.1 results of software analysis. The distribution frequency of SNPs locus genotype of NPR3 gene in case and control group was tested by Hardy-Weinberg equilibrium test. The statistical software was SPSS18. .0 software. T test, chi-square test and binary Logistic regression analysis were used for statistical analysis and bilateral test. P0.05 was statistically significant. Haploview4.2 program was used for linkage disequilibrium analysis. Results the Hardy-Weinberg balance test was chi-square test. The 9 SNPs were all P 0. 05 in the case group and the control group. The SNPs genotype of NPR3 gene in case group and control group was analyzed by binary Logistic regression analysis. The risk of hypertension in rs1173773GG genotype carriers was increased by 95 CI 3.164U 1.011-9.903 P value 0.037. After adjusting for age, sex, body mass index, waist circumference, smoking history, drinking history and family history of hypertension, OR was 95 CI 3.0121.002-9.728). P value was 0. 041, which was still statistically significant. The other 8 SNPs were not found in the two groups. The allele genotypes of the two groups were compared with each other by chi-square test. There was no significant difference in P value (P = 0.05). Further comparison was made between BMI "g _ 25kg / m ~ 2 and BMI25Kg/m2 in hypertension group." The risk of overweight or obesity in GG genotype carriers was higher than that in GG genotype carriers. The OR value of GG genotype carriers was 95CI3.509 ~ 0.935-13.176 (P = 0.049), which was statistically significant. There was no significant difference in the G / A allele carrying rate (P 0.05). In addition, 113 patients with type 2 diabetes were compared in the hypertension group whether or not they were associated with type 2 diabetes. There was no significant difference in the distribution of G / A allele genotypes. Conclusion this study found that G- A alleles were found in Chinese Han population. The risk of essential hypertension was increased in NPR3 rs1173773 GG carriers. The risk of essential hypertension associated with overweight or obesity also increased.
【学位授予单位】：宁波大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R544.11

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