重组人脑钠尿肽对心力衰竭大鼠心肌重构及缝隙链接蛋白43表达的影响

发布时间：2018-01-30 23:47

本文关键词： 心力衰竭重组人脑利钠肽缝隙连接链接蛋白心肌重构　出处：《山东医药》2017年33期 　论文类型：期刊论文

【摘要】：目的探讨重组人脑钠尿肽(rhBNP)对压力超负荷所致心力衰竭大鼠心肌重构及缝隙链接蛋白43(Cx43)表达的影响。方法将45只雄性SD大鼠随机分为心力衰竭(F)组、心力衰竭+rhBNP(B)组、假手术(SO)组各15只,F组、B组经腹主动脉缩窄法建立压力超负荷心力衰竭大鼠模型,SO组大鼠不缩窄腹主动脉。B组经腹腔皮下注射用生理盐水配制的rhBNP 25μg/(kg·d),SO组和F组仅用生理盐水腹腔皮下注射,均给药8周。检测各组左心功能、血流动力学、QT离散度、左室心肌组织病理形态、心肌纤维化沉积和心肌Cx43的表达。结果与SO组比较,F组左心功能、血流动力学、QT离散度、左室心肌组织病理形态、心肌纤维化沉积和心肌Cx43表达均有统计学差异(P均0.05),显示心力衰竭模型制作成功。与F组比较,B组大鼠平均动脉压下降、QT离散度减小、心肌细胞排列紊乱程度减轻、心肌细胞间胶原纤维沉积减少、Cx43表达增加(P均0.05)。结论rh BNP可改善压力超负荷下心力衰竭大鼠的血流动力学指标,上调心肌组织Cx43表达并抑制心肌重构。
[Abstract]:Objective to investigate the effects of recombinant human brain natriuretic peptide (rhBNP) on myocardial remodeling and gap link protein 43 Cx43 in rats with congestive heart failure induced by pressure overload. Methods 45 male Sprague-Dawley rats were randomly divided into two groups: heart failure group (F). The rats with congestive heart failure (rhBNPB) group and sham operation group (15 rats in each group) were induced by abdominal aorta coarctation. RhBNP 25 渭 g / kg 路d ~ (-1) prepared by normal saline was injected subcutaneously into the abdominal cavity of rats of group so and group F and group F were only injected subcutaneously with normal saline. The left ventricular function, hemodynamics and QT dispersion, left ventricular histopathology, myocardial fibrosis deposition and myocardial Cx43 expression were measured in each group for 8 weeks. Results the results were compared with those in so group. In group F, there were significant differences in left ventricular function, hemodynamics and QT dispersion, left ventricular histopathology, myocardial fibrosis deposition and myocardial Cx43 expression (P < 0.05). The results showed that the model of heart failure was made successfully. Compared with group F, the mean arterial pressure decreased and the dispersion of QT decreased, the degree of myocardial cell arrangement disorder decreased, and the deposition of collagen fibers between myocardial cells decreased in group B. Conclusion Rh BNP can improve the hemodynamic parameters, up-regulate the expression of Cx43 and inhibit myocardial remodeling in rats with congestive heart failure under pressure overload.
【作者单位】：南华大学附属第一医院;南华大学附属第二医院;
【基金】：国家自然科学基金资助项目(81202830)
【分类号】：R541.6
【正文快照】： 脑利钠肽是心力衰竭诊断、疗效评估及预后判断的一项有效指标。但是,关于脑利钠肽对心力衰竭时的缝隙连接以及心肌重构的作用及其机制尚不明确。缝隙连接是心肌细胞之间一种电化学偶联的通道,其与心室重构有着密切的关系,其基本构成单位是缝隙连接蛋白(Cx),而Cx43则是心肌工作

【相似文献】