PM2.5对心血管病人血清TNF-α、IL-2、IL-8以及ICAM-1水平影响的研究

发布时间：2018-02-14 00:15

本文关键词： PM2.5 心血管病事件 TNF-α IL-2 IL-8 ICAM-1　出处：《吉林大学》2017年硕士论文　论文类型：学位论文

【摘要】：研究背景:大量流行病学研究发现长期或短期暴露于PM2.5均导致心血管事件明显增加。同时也有许多动物及健康人群实验研究证实暴露于PM2.5后机体血清炎症因子如TNF-α、IL-2、IL-8以及ICAM-1水平显著升高。普遍认为PM2.5引起机体炎症因子表达增加是其导致心血管事件发生率增加的主要机制。研究目的:本研究旨在明确PM2.5对心血管病人血清TNF-α、IL-2、IL-8、ICAM-1表达水平的影响,同时比较以不同浓度PM2.5对心血管病人血清相关炎症因子表达水平影响的差异。系统阐述PM2.5对心血管系统的损害的可能机制,阐述上述炎症因子的致动脉粥样硬化作用,为研究新型抗动脉粥样硬化药物提供一定的理论支持。研究方法:纳入我院心内科的心血管病病人共92名,收集病人的中性粒细胞计数(NEUT)、单核细胞计数(MONO)、淋巴细胞计数(LYM)、血小板计数(PLT)、纤维蛋白定量(FIB)、血肌酐(CREA)以及谷丙转氨酶(ALT)。应用酶联免疫吸附法(ELISA)测定所有对象入院时的血清TNF-α、IL-2、IL-8以及ICAM-1的浓度。记录病人入院前的PM2.5浓度值,根据PM2.5浓度值将调查对象分为空气质量优良A组,空气轻度污染B组,空气中重度污染C组,应用SPSS 17.0分析数据,A、B、C三组比较时检验水准为0.05,进一步两两比较时检验水准为0.017。研究结果:A组、B组、C组对象血清LYM、FIB、TNF-α、IL-2、IL-8以及ICAM-1水平有显著差异(P0.05)。C组血清TNF-α及IL-8浓度均显著高于A组及B组(P0.017);但A、B组无显著差异(P0.017)。C组血清IL-2及ICAM-1浓度高于B组(P0.017),B组高于A组(P0.017),差异具有统计学意义。C组LYM计数高于B组(P0.017),A组与B组LYM无明显差异。A组与C组间差异无统计学意义。C组FIB定量显著高于A组(P0.017),差异有统计学意义。A组与B组、B组与C组间差异不明显(P0.017)。研究结论:暴露于PM2.5可能导致心血管病人LYM、FIB、TNF-α、IL-2、IL-8以及ICAM-1表达水平显著增加。PM2.5浓度愈高则其对血清炎症介质的表达水平影响愈显著。PM2.5可能要达到一定浓度才对暴露于其中的个体的血清炎症因子水平产生显著的影响。
[Abstract]:Background: a large number of epidemiological studies have found that long-term or short-term exposure to PM2.5 leads to a significant increase in cardiovascular events. At the same time, many animal and healthy population studies have confirmed that the exposure to PM2.5 after the body's serum inflammatory factors such as. The levels of TNF- 伪 IL-2IL-8 and ICAM-1 were significantly increased. It is generally believed that the increase in the expression of inflammatory factors induced by PM2.5 is the main mechanism leading to an increase in the incidence of cardiovascular events. Objective: the purpose of this study was to determine the effect of PM2.5 on serum TNF- 伪 IL-2IL-8ICAM-1 in cardiovascular patients. The effect of the level of expression, At the same time, the effects of different concentrations of PM2.5 on the expression of serum related inflammatory factors in cardiovascular patients were compared. The possible mechanism of PM2.5 damage to the cardiovascular system and the atherogenic effect of the above mentioned inflammatory factors were expounded. To provide some theoretical support for the study of new anti-atherosclerotic drugs. Methods: 92 patients with cardiovascular diseases were included in our hospital. Neutrophil count, monocyte count, monocyte count, lymphocyte count, platelet count, fibrin quantitative FIBT, creatinine creatinine (creatinine) and alt were collected. Elisa was used to detect all the patients. Serum levels of TNF- 伪 IL-2IL-8 and ICAM-1 were recorded before admission, and the concentrations of PM2.5 before admission were recorded. According to the concentration of PM2.5, the subjects were divided into good air quality group A, mild air pollution group B, air moderate and severe pollution group C. Using SPSS 17.0 analysis data, the test level was 0.05 in the three groups and 0.017 in the other two groups. The results showed that the serum levels of TNF- 伪, IL-2IL-8 and ICAM-1 in group C were significantly different from those in group C (P0.05.C). The results showed that the levels of TNF- 伪 and IL-8 in group C were significantly higher than those in group B (P < 0.05). The results showed that the levels of TNF- 伪 and IL-8 in group C were significantly higher than those in group B (P < 0.05). The serum IL-2 and ICAM-1 levels in group A were higher than those in group B (P 0.017), and the LYM count in group C was higher than that in group A (P 0.017 7). The LYM count in group C was higher than that in group B (P 0.017). There was no significant difference between group A and group C. the LYM count in group C was significantly higher than that in group B (P 0.017). There was no significant difference between group A and group C. There was no significant difference between the two groups. The quantitative FIB of group C was significantly higher than that of group A (P 0.017). There was no significant difference between group A and group B (P 0.017). Conclusion: exposure to PM2.5 may lead to the cardiovascular patient LYMM-FIBTNF- 伪 TNF-2 IL-8 and ICAM-1 table. The higher the concentration of PM2.5, the more significant the effect of PM2.5 on the expression of serum inflammatory mediators. The higher the concentration of PM2.5 may be, the higher the concentration of PM2.5 may have a significant effect on the level of serum inflammatory cytokines of the exposed individuals.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R54

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