肿瘤坏死因子-α诱导连接蛋白43重构在心力衰竭大鼠室性心律失常发生中的作用

发布时间：2018-02-15 13:05

本文关键词： 心力衰竭肿瘤坏死因子-α 连接蛋白室性心律失常　出处：《免疫学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的观察肿瘤坏死因子-α(TNF-α)对心力衰竭大鼠连接蛋白(Cx)43表达和分布调控作用,并探讨其与室性心律失常发生的关系。方法 36只SD大鼠随机分为3组,每组12只:心衰组(F组)、加药组(D组)、假手术组(S组)。通过腹主动脉缩窄法构建心力衰竭模型,D组待术后第2周开始应用TNF-α螯合剂rhTNFR:Fc至第16周。术后第16周应用程序刺激诱发室性心律失常,记录各组诱发情况。Western blot检测心肌组织TNF-α、总CX43(T-Cx43)、磷酸化CX43(P-Cx43)和非磷酸化CX43(NP-Cx43)的蛋白表达水平,应用激光共聚焦显微镜观察T-Cx43的分布情况。结果与S组相比,F组心肌组织TNF-α表达显著增加(P0.05),T-Cx43表达下降且分布紊乱,P-Cx43水平下降且NP-Cx43水平增加(P0.05),室性心律失常诱发率明显升高(P0.05);与F组比较,D组TNF-α表达显著减少(P0.05),T-Cx43和P-Cx43水平有所增加(P0.05),NP-Cx43水平下降(P0.05),T-Cx43分布紊乱有所减轻,室性心律失常诱发率显著下降(P0.05)。结论心力衰竭大鼠心肌组织中过表达的TNF-α可以诱导Cx43重构,其在心衰室性心律失常的发生中可能发挥重要作用。
[Abstract]:Objective to observe the effect of TNF- 伪 on the expression and distribution of connexin 43 in rats with heart failure, and to explore the relationship between TNF- 伪 and ventricular arrhythmia.Methods Thirty-six SD rats were randomly divided into three groups. 12 rats in each group were treated with TNF- 伪 chelator rhTNFR:Fc from the 2nd week to the 16th week after operation. Program stimulation induces ventricular arrhythmia, The expression levels of TNF- 伪, total CX43 T-Cx43, phosphorylated CX43 P-Cx43 and non-phosphorylated CX43 NP-Cx43) were measured by Western blot. Results compared with group S, the expression of TNF- 伪 in myocardial tissue of group F increased significantly, the expression of T-Cx43 decreased, the level of P-Cx43 decreased, the level of NP-Cx43 increased, and the rate of ventricular arrhythmia was induced. Compared with group F, the expression of TNF- 伪 in group D decreased significantly, and the levels of T-Cx43 and P-Cx43 increased. Conclusion the overexpression of TNF- 伪 in myocardial tissue of heart failure rats can induce Cx43 remodeling, which may play an important role in the occurrence of ventricular arrhythmias in heart failure rats.
【作者单位】：承德医学院附属医院重症医学科;华中科技大学同济医学院附属协和医院心内科;
【基金】：国家自然科学基金(30971242)
【分类号】：R541

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