GP78基因多态性与冠心病的关联性研究

发布时间：2018-02-16 17:13

  本文关键词： 冠心病 GP78 单核苷酸多态性 病例对照研究　出处：《新疆医科大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的：探讨GP78基因多态性与冠心病的相关性。方法：选择维吾尔族和汉族两个人群分别进行病例对照研究,汉族人群：选择经冠脉造影检查确诊的冠心病患者602例和性别、年龄、民族均匹配的正常健康对照人群572例；维族人群：选择经冠脉造影检查确诊的冠心病患者374例和性别、年龄、民族均匹配的正常健康对照人群376例,选择GP78基因的3个SNPs (rs731119, rs2617849 and rs2440472),采用实时荧光定量PCR (TaqMan)方法对GP78基因进行基因分型,数据分为三组：整体组、男性组,女性组。结果：在汉族人群中,对于整体,GP78基因的rs2440472的基因型分布在冠心病组和健康对照组间有显著差异(P=0.008),对于整体组和男性组,SNP3 (rs2440472)的等位基因分布、显性模型(AA VS AG+GG)以及隐性模型(GG VS AG+AA)在冠心病组和健康对照组之间有显著差异(等位基因：P=0.003vsP=0.002；显性模型：P=0.041vsP=0.026；隐形模型：P=0.004vs0.004)。在调整‘主要危险因素的干扰后,Logistic回归分析显示：对于整体组和男性组,显性模型(AA VS AG+GG)和隐形模型(GG VS AG+AA)分布在冠心病组和健康对照组间仍然均有显著差异(显性模型整体：OR:0.760,95% CI:0.584-0.99, P=0.042;显性模型男性组：OR:0.686,95% CI:0.498-0.946, P=0.022;隐形模型整体OR:1.451,95% CI:1.067-1.974, P=0.018;隐形模型男性组：OR:1.789,95% CI:1.219-2.627).在维吾尔族人群中,GP78基因的3个SNPs (rs731119, rs2617849 and rs2440472)的基因型及等位基因分布频率在冠心病组和对照组间均无显著差异(P均0.05)。结论：GP78基因rs2440472的GG基因型和G等位基因可能是汉族人群发生冠心病的危险因素,可做为冠心病的易感基因标记。而AA基因型和A等位基因可能是汉族人群发生冠心病的保护因素。
[Abstract]:Objective: to investigate the relationship between GP78 gene polymorphism and coronary heart disease (CHD). Methods: a case-control study was conducted in two populations of Uygur and Han nationality. 572 healthy controls matched by age and nationality, 374 patients with coronary heart disease confirmed by coronary angiography, 376 healthy controls matched by age and nationality were selected. Three SNPs rs731119 and rs2617849 and rs2440472 of GP78 gene were selected, and the genotyping of GP78 gene was carried out by real-time fluorescent quantitative PCR Taq Man. the data were divided into three groups: whole group, male group and female group. There was a significant difference in the genotype distribution of rs2440472 between CHD group and healthy control group, and the allele distribution of SNP3 rs2440472 in whole group and male group. There were significant differences between the dominant model (AA vs AG GGG) and the recessive model (GG vs AG AAA) between the coronary heart disease group and the healthy control group (allele: 1: 0. 003 vs P0. 002; the dominant model: P0. 041 vs P0. 026; the invisible model: P0. 004 vs 0. 004. after adjusting for the interference of 'main risk factors), logistic regression analysis was performed. For the whole group and the male group, There were still significant differences in the distribution of AA-VS AG GGG and GG VS AG AAA between coronary heart disease group and healthy control group (dominant model: OR0.76095% CI: 0.584-0.99, P0. 042; dominant male group: 0. 68695% CIW 0. 498-0.946, P0. 022; occult model: OR1.45195%: CI1.067-1.974, Pu. 0.018; male: ORW: 0. 68695% CIW 0. 498-0.946; occult model: OR1.45195%: CI1.067-1.974, P0. 018; male: 1. 68695% CI: 0. 498-0. 022; There were no significant differences in genotype and allele frequencies of three SNPs rs731119 and rs2617849 and rs2440472 of GP78 gene between coronary heart disease group and control group. Conclusion there is no significant difference in GG genotype and allele frequency between coronary heart disease group and control group. Conclusion the GG genotypes and alleles of rs2617849 GP78 gene rs2440472 have no significant difference between coronary heart disease group and control group. Conclusion there is no significant difference in GG genotype and allele frequency between coronary heart disease group and control group. G allele may be the risk factor of coronary heart disease in Han population. AA genotype and A allele may be the protective factors of coronary heart disease in Han population.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R541.4
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本文编号：1516027