骨桥蛋白对压力过负荷心衰小鼠的影响

发布时间：2018-02-21 21:41

本文关键词： 小鼠过负荷心衰骨架蛋白　出处：《中国老年学杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的同步观察心肌骨桥蛋白(OTN)、血清氨基末端B型利钠肽前体(NT-proBNP)在过负荷心衰(HF)、过负荷心衰重置于正常负荷小鼠心脏的影响。方法选用雄性昆明种系小鼠C57BL/6野生型小鼠,分为Sham组、过负荷HF组、过负荷心衰重新置于正常负荷(HF-NL)组,观察三组形态学改变、血清NT-proBNP浓度以及OPN mRNA表达。结果光镜下Sham组小鼠心肌细胞结构完整,肌纤维排列整齐;过负荷HF组小鼠心肌纤维间隙增宽,心肌细胞呈不同程度变性及肥大,部分心肌细胞呈局灶性坏死;HF-NL组与过负荷HF组比较,心肌纤维间隙增宽得到改善,心肌细胞变性、肥大以及局灶性坏死变轻;血清NT-proBNP浓度在Sham组、过负荷HF组、HF-NL组分别为(54.10±8.91)mg/L、(459.78±22.79)mg/L(与Sham组比较,P0.01)、(362.67±43.73)mg/L(与过负荷HF组比较,P0.01)。OPN mRNA表达在正常小鼠中的表达较弱〔(0.95±0.10)%〕;在过负荷HF组增强〔(3.38±0.12)%,与Sham组比较,P0.01〕,而在HF-NL组表达减轻〔(1.97±0.14)%,与过负荷HF组比较,P0.01〕。结论OPN参与过负荷心衰置于正常负荷后的心肌重塑过程,抑制OPN表达可能是减轻、减缓过负荷HF发生、发展的干预靶点之一。
[Abstract]:Objective to observe the effects of OTNN, NT-proBNPs, a precursor of serum amino-terminal B-type natriuretic peptide (NT-proBNPN), on the heart of mice with congestive heart failure (CHF) and its resetting to normal load mice. Methods male Kunming strain mice, C57BL / 6 wild-type mice, were used in this study. Sham group, HF group and HF-NL group were divided into three groups. Morphological changes, serum NT-proBNP concentration and OPN mRNA expression were observed in the three groups. Results under light microscope, the myocardial cell structure of Sham group was intact and the muscle fibers were arranged neatly. Compared with overload HF group, myocardial fiber gap widened, myocardial cells denatured and hypertrophic, and some myocardial cells showed focal necrosis. Compared with overload HF group, myocardial fiber gap widened and myocardial cell denaturation was improved in HF-NL group. Hypertrophy and focal necrosis became mild, serum NT-proBNP concentration in Sham group, The HF-NL group was 54.10 卤8.91 mg / L (459.78 卤22.79 mg / L vs Sham group, 362.67 卤43.73 mg / L respectively) (compared with the overloaded HF group, the expression of P0.01 .OPN mRNA was weaker in normal mice than that in the overload HF group (0.95 卤0.10); in the overload HF group, it increased 3.38 卤0.12g / L; compared with that in the Sham group, P 0.01g / L; in the HF-NL group, the expression was reduced by 1.97 卤0.14), and that in the overload HF group was increased by 3.38 卤0.12g / L, compared with that in the Sham group, while in the HF-NL group, the expression of P0.01 / L was reduced to 1.97 卤0.14. Conclusion OPN is involved in myocardial remodeling after overload in patients with heart failure after normal load. Inhibition of OPN expression may be one of the intervention targets to alleviate, slow down the occurrence and development of overload HF.
【作者单位】：西北民族大学附属第一医院宁夏人民医院心胸外科;上海交通大学附属新华医院心胸外科;吉林大学第一医院心脏外科;
【分类号】：R54

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